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Titolo:
CHEMORECEPTORS EXPRESSED IN TASTE, OLFACTORY AND MALE REPRODUCTIVE TISSUES
Autore:
THOMAS MB; HAINES SL; AKESON RA;
Indirizzi:
DUKE UNIV,MED CTR,DEPT NEUROBIOL,BRYAN RES BLDG DURHAM NC 27710 CHILDRENS HOSP RES FDN,DEPT DEV BIOL CINCINNATI OH 45229
Titolo Testata:
Gene
fascicolo: 1-2, volume: 178, anno: 1996,
pagine: 1 - 5
SICI:
0378-1119(1996)178:1-2<1:CEITOA>2.0.ZU;2-A
Fonte:
ISI
Lingua:
ENG
Soggetto:
ODORANT RECEPTOR EXPRESSION; PROTEIN-COUPLED RECEPTORS; MULTIGENE FAMILY; GENE FAMILY; EPITHELIUM; CELL; RAT; ORGANIZATION; INFORMATION; RHODOPSIN;
Keywords:
7 TRANSMEMBRANE DOMAIN RECEPTORS; REVERSE TRANSCRIPTASE-POLYMERASE CHAIN REACTION; RECOMBINANT DNA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
25
Recensione:
Indirizzi per estratti:
Citazione:
M.B. Thomas et al., "CHEMORECEPTORS EXPRESSED IN TASTE, OLFACTORY AND MALE REPRODUCTIVE TISSUES", Gene, 178(1-2), 1996, pp. 1-5

Abstract

We have identified three genes encoding previously uncharacterized chemoreceptors expressed in rat sensory and reproductive tissues using areverse transcriptase polymerase chain reaction strategy. Degenerate oligonucleotides designed from conserved sequences in the rat olfactory receptor gene family were used to amplify candidate receptor gene products expressed in taste tissue. Sequence analysis of three distinct clonal isolates revealed that the gene products from taste bud were 30-75% identical to previously identified olfactory receptor genes. The genomic coding sequences predicted protein structures with seven membrane spanning regions that have strong conservation relative to other members of the G-protein-coupled olfactory receptor gene family. Transcripts for each of the three gene products were detected exclusively intaste, olfactory and male reproductive tissue. Sequence analysis of the polymerase chain reaction products confirmed that identical transcripts were expressed in all three tissues. These findings are the firstdemonstration that identical olfactory receptor-like gene are expressed in three distinct tissues.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 10/07/20 alle ore 11:57:38