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Titolo:
RAPID DIAGNOSIS OF METHICILLIN-RESISTANT STAPHYLOCOCCUS-AUREUS BACTEREMIA BY NESTED POLYMERASE CHAIN-REACTION
Autore:
KITAGAWA Y; UEDA M; ANDO N; ENDO M; ISHIBIKI K; KOBAYASHI Y; ARAI T; KITAJIMA M;
Indirizzi:
KEIO UNIV,DEPT SURG,SHINJUKU KU,35 SHINANOMACHI TOKYO 160 JAPAN KEIO UNIV HOSP,DEPT SURG TOKYO JAPAN KEIO UNIV HOSP,DEPT LAB MED TOKYO JAPAN MEIJI COLL PHARM,DEPT MICROBIOL TOKYO 154 JAPAN
Titolo Testata:
Annals of surgery
fascicolo: 5, volume: 224, anno: 1996,
pagine: 665 - 671
SICI:
0003-4932(1996)224:5<665:RDOMSB>2.0.ZU;2-C
Fonte:
ISI
Lingua:
ENG
Soggetto:
PENICILLIN-BINDING PROTEIN; BETA-LACTAM ANTIBIOTICS; GENE; DNA; IDENTIFICATION; EPIDERMIDIS; AFFINITY; SEQUENCE; CLONING; BLOOD;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
30
Recensione:
Indirizzi per estratti:
Citazione:
Y. Kitagawa et al., "RAPID DIAGNOSIS OF METHICILLIN-RESISTANT STAPHYLOCOCCUS-AUREUS BACTEREMIA BY NESTED POLYMERASE CHAIN-REACTION", Annals of surgery, 224(5), 1996, pp. 665-671

Abstract

Objective The purpose of this study was to establish a rapid and sensitive diagnostic method for methicillin-resistant Staphylococcus aureus (MRSA) bacteremia in postoperative patients. Summary Background DataAs a result of diffusion and abuse of third-generation cephalosporin antibiotics in the 1980s in Japan, an outbreak of MRSA infection has been posed. in the field of surgery, severe postoperative infections with MRSA such as MRSA bacteremia, which may lead to multiple organ failure, have emerged with a high mortality. Methods Thirty-five patients with high fever (above 38.5 C) or watery diarrhea or both within 2 weeks after gastrointestinal major surgery and 6 healthy volunteers were examined. Nested polymerase chain reaction was used to detect mecA andtoxic shock syndrome toxin-1 (TSST-1) genes in blood specimens. Results The mecA and TSST-1 genes were not detected in the blood samples ofany of the six healthy volunteers. In all 12 samples from which,MRSA colonies were isolated by blood culture, mecA and TSST-1 genes were detected. Although it took at least 48 hours to identify MRSA by the blood culture method, the presence of mecA and TSST-1 genes was determined by nested polymerase chain reaction method within only 3 to 4 hours after blood sampling. Conclusions This method, as a sensitive and rapid monitoring system for MRSA bacteremia, would be clinically beneficial for prevention of cross infection and for early determination of appropriate treatment for infected patients.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 20/01/21 alle ore 11:24:42