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Titolo:
A TEST OF THE ROLE OF THE PROXIMAL HISTIDINES IN THE PERUTZ MODEL FORCOOPERATIVITY IN HEMOGLOBIN
Autore:
BARRICK D; HO NT; SIMPLACEANU V; DAHLQUIST FW; HO C;
Indirizzi:
JOHNS HOPKINS UNIV,SCH MED,DEPT BIOPHYS & BIOPHYS CHEM,725 N WOLFE STBALTIMORE MD 21205 UNIV OREGON,INST MOL BIOL EUGENE OR 97403 CARNEGIE MELLON UNIV,DEPT BIOL SCI PITTSBURGH PA 15213
Titolo Testata:
Nature structural biology
fascicolo: 1, volume: 4, anno: 1997,
pagine: 78 - 83
SICI:
1072-8368(1997)4:1<78:ATOTRO>2.0.ZU;2-T
Fonte:
ISI
Lingua:
ENG
Soggetto:
NUCLEAR-MAGNETIC-RESONANCE; HUMAN ADULT HEMOGLOBIN; LIGAND-BINDING; M-BOSTON; HEME; REPLACEMENT; MECHANISM; MYOGLOBIN; DYNAMICS; MUTANT;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
49
Recensione:
Indirizzi per estratti:
Citazione:
D. Barrick et al., "A TEST OF THE ROLE OF THE PROXIMAL HISTIDINES IN THE PERUTZ MODEL FORCOOPERATIVITY IN HEMOGLOBIN", Nature structural biology, 4(1), 1997, pp. 78-83

Abstract

Human haemoglobin has long been a paradigm for cooperative ligand binding and allostery. Through analysis of the crystal structures of deoxyhaemoglobin and liganded haemoglobin, Perutz proposed a model for cooperativity in which the bond between the proximal histidine and the protein couples haem rearrangements to protein structure rearrangements. Here we test this model by deleting the bonds between the histidine imidazole ride chain and the polypeptide, This detachment method allowsus to determine directly the contribution of proximal histidine coupling to cooperativity of distal ligand binding, Proximal detachment significantly increases ligand affinity reduces cooperativity, and prevents quaternary structure switching, in accord with the Perutz model. Residual cooperativity indicates that additional haem communication pathways exist that do not involve the proximal histidine coupling mechanism.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 13/07/20 alle ore 18:05:23