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Titolo:
AN EXTRACELLULAR SIGNALING COMPONENT IN PROPAGATION OF ASTROCYTIC CALCIUM WAVES
Autore:
HASSINGER TD; GUTHRIE PB; ATKINSON PB; BENNETT MVL; KATER SB;
Indirizzi:
UNIV UTAH,DEPT ANAT & NEUROBIOL SALT LAKE CITY UT 84132 UNIV UTAH,DEPT ANAT & NEUROBIOL SALT LAKE CITY UT 84132 ALBERT EINSTEIN COLL MED,DEPT NEUROSCI BRONX NY 10461
Titolo Testata:
Proceedings of the National Academy of Sciences of the United Statesof America
fascicolo: 23, volume: 93, anno: 1996,
pagine: 13268 - 13273
SICI:
0027-8424(1996)93:23<13268:AESCIP>2.0.ZU;2-W
Fonte:
ISI
Lingua:
ENG
Soggetto:
MECHANICAL STIMULATION; GLIAL-CELLS; GLUTAMATE; NEURONS;
Keywords:
GLIA; ASTROCYTES; CALCIUM WAVES; EXTRACELLULAR SIGNAL;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
18
Recensione:
Indirizzi per estratti:
Citazione:
T.D. Hassinger et al., "AN EXTRACELLULAR SIGNALING COMPONENT IN PROPAGATION OF ASTROCYTIC CALCIUM WAVES", Proceedings of the National Academy of Sciences of the United Statesof America, 93(23), 1996, pp. 13268-13273

Abstract

Focally evoked calcium waves in astrocyte cultures have been thought to propagate by gap-junction-mediated intercellular passage of chemical signal(s). In contrast to this mechanism we observed isolated astrocytes, which had no physical contact with other astrocytes in the culture, participating in a calcium wave. This observation requires an extracellular route of astrocyte signaling. To directly test for extracellular signaling we made cell-free lanes 10-300 mu m wide in confluent cultures by deleting astrocytes with a glass pipette, After 4-8 hr of recovery, regions of confluent astrocytes separated by lanes devoid of cells were easily located, Electrical stimulation was used to initiatecalcium waves. Waves crossed narrow (<120 mu m) cell-free lanes in 15of 36 cases, but failed to cross lanes wider than 120 mu m in eight of eight cases, The probability of crossing narrow lanes was not correlated with the distance from the stimulation site, suggesting that cells along the path of the calcium wave release the extracellular messenger(s). Calculated velocity across the acellular lanes was not significantly different from velocity through regions of confluent astrocytes. Focal superfusion altered both the extent and the direction of calcium waves in confluent regions, These data indicate that extracellular signals may play a role in astrocyte-astrocyte communication in situ.

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Documento generato il 15/07/20 alle ore 08:30:59