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Titolo:
DEVELOPMENT OF PILUS ORGANELLE SUBASSEMBLIES IN-VITRO DEPENDS ON CHAPERONE UNCAPPING OF A BETA-ZIPPER
Autore:
BULLITT E; JONES CH; STRIKER R; SOTO G; JACOBDUBUISSON F; PINKNER J; WICK MJ; MAKOWSKI L; HULTGREN SJ;
Indirizzi:
BOSTON UNIV,SCH MED,DEPT BIOPHYS BOSTON MA 02118 BOSTON UNIV,SCH MED,DEPT BIOPHYS BOSTON MA 02118 WASHINGTON UNIV,SCH MED,DEPT MOL MICROBIOL ST LOUIS MO 63110 FLORIDA STATE UNIV,INST MOL BIOPHYS TALLAHASSEE FL 32306
Titolo Testata:
Proceedings of the National Academy of Sciences of the United Statesof America
fascicolo: 23, volume: 93, anno: 1996,
pagine: 12890 - 12895
SICI:
0027-8424(1996)93:23<12890:DOPOSI>2.0.ZU;2-A
Fonte:
ISI
Lingua:
ENG
Soggetto:
UROPATHOGENIC ESCHERICHIA-COLI; PAPD CHAPERONE; SUBUNIT RECOGNITION; ADHESIN; BIOGENESIS; COMPLEXES; FIMBRIAE; REVEALS; BINDING;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
22
Recensione:
Indirizzi per estratti:
Citazione:
E. Bullitt et al., "DEVELOPMENT OF PILUS ORGANELLE SUBASSEMBLIES IN-VITRO DEPENDS ON CHAPERONE UNCAPPING OF A BETA-ZIPPER", Proceedings of the National Academy of Sciences of the United Statesof America, 93(23), 1996, pp. 12890-12895

Abstract

The major subassemblies of virulence-associated P pili, the pilus rod(comprised of PapA) and tip fibrillum (comprised of PapE), were reconstituted from purified chaperone-subunit complexes in vitro. Subunits are held in assembly-competent conformations in chaperone-subunit complexes prior to their assembly into mature pili. The PapD chaperone binds, in part, to a conserved moth present at the C terminus of the subunits via a beta zippering interaction. Amino acid residues in this conserved motif were also found to be essential for subunit-subunit interactions necessary for the formation of pill, thus revealing a molecular mechanism whereby the PapD chaperone may prevent premature subunit-subunit interactions in the periplasm. Uncapping of the chaperone-protected C terminus of PapA and PapE was mimicked in vitro by freeze-thaw techniques and resulted in the formation of pilus rods and tip fibrillae, respectively. A mutation in the leading edge of the beta zipper ofPapA produces pilus rods with an altered helical symmetry and azimuthal disorder. This change in the number of subunits per turn of the helix most likely reflects involvement of the leading edge of the beta zipper in forming a right-handed helical cylinder, Organelle developmentis a fundamental process in all living cells, and these studies shed new light on how immunoglobulin-like chaperones govern the formation of virulence-associated organelles in pathogenic bacteria.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/12/20 alle ore 10:57:15