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Titolo:
IMMUNOBLOT ANALYSIS OF URINARY FIBRONECTIN OF NORMAL AND PASSIVE HEYMANN NEPHRITIS RATS
Autore:
NISHIZAWA Y; FUKAI F; NATORI Y; SHIMATANI K; KATAYAMA T;
Indirizzi:
SCI UNIV TOKYO,FAC PHARMACEUT SCI,DEPT PATHOPHYSIOL,SHINJUKU KU TOKYO162 JAPAN SCI UNIV TOKYO,FAC PHARMACEUT SCI,DEPT PATHOPHYSIOL,SHINJUKU KU TOKYO162 JAPAN SANTEN PHARMACEUT CO LTD,CLIN RES & DEV DEPT,DEV DIV,MINATO KU TOKYO 106 JAPAN INT MED CTR JAPAN,RES INST,DIV PATHOPHYSIOL,SHINJUKU KU TOKYO JAPAN
Titolo Testata:
Journal of clinical biochemistry and nutrition
fascicolo: 3, volume: 20, anno: 1996,
pagine: 231 - 239
SICI:
0912-0009(1996)20:3<231:IAOUFO>2.0.ZU;2-U
Fonte:
ISI
Lingua:
ENG
Soggetto:
IMMUNOENZYMOMETRIC ASSAY; MONOCLONAL-ANTIBODIES; PROTEINS; CELLS; GLYCOPROTEIN; PROTEOLYSIS; FIBROBLASTS; DISEASE; CANCER; KIDNEY;
Keywords:
FIBRONECTIN; FIBRONECTIN FRAGMENTS; IMMUNOBLOT ANALYSIS; URINE; NEPHRITIS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
29
Recensione:
Indirizzi per estratti:
Citazione:
Y. Nishizawa et al., "IMMUNOBLOT ANALYSIS OF URINARY FIBRONECTIN OF NORMAL AND PASSIVE HEYMANN NEPHRITIS RATS", Journal of clinical biochemistry and nutrition, 20(3), 1996, pp. 231-239

Abstract

Immunoblot analysis for urinary fibronectin (FN) of normal and passive Heymann nephritis (PHN) rats was performed. When 2-5% skim milk or 2-10% bovine serum albumin solution was used as a blocking buffer for the immunoblot analysis, remarkable nonspecific interactions were observed between many urinary proteins and immunoglobulins used as a primary as well as a secondary antibody. Blocking of the trnasblot membrane with a 2% gamma-globulin solution prevented such nonspecific interactions, As a result, two FN fragments, not intact FN, with molecular masses of 55- and 65-kDa were detected in all the normal rat urine samples. In PHN rat urine, intact FN and several FN fragments of 100-200-kDa were evident instead of the 55- and 65-kDa FN fragments. These resultsindicate that metabolism of FN and excretion of the FN metabolites into urine may be changed during the development of the renal disease.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/09/20 alle ore 21:49:08