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Titolo:
IDENTIFICATION OF AN ALTERNATIVELY SPLICED TRANSCRIPT OF EQUINE INTERLEUKIN-1-BETA
Autore:
KATO H; YOUN HY; OHASHI T; WATARI T; GOITSUKA R; TSUJIMOTO H; HASEGAWA A;
Indirizzi:
UNIV TOKYO,FAC AGR,DEPT VET INTERNAL MED,BUNKYO KU,1-1-1 YAYOI TOKYO 113 JAPAN
Titolo Testata:
Gene
fascicolo: 1-2, volume: 177, anno: 1996,
pagine: 11 - 16
SICI:
0378-1119(1996)177:1-2<11:IOAAST>2.0.ZU;2-W
Fonte:
ISI
Lingua:
ENG
Soggetto:
MESSENGER-RNA; CLONING; EXPRESSION; SEQUENCE; RECEPTOR; CDNA; MACROPHAGES; PRECURSOR; BINDING; GENE;
Keywords:
ALTERNATIVE SPLICING; HORSE; INTERLEUKIN-1-BETA; CYTOKINE; NUCLEOTIDE SEQUENCE; MESSENGER-RNA EXPRESSION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
21
Recensione:
Indirizzi per estratti:
Citazione:
H. Kato et al., "IDENTIFICATION OF AN ALTERNATIVELY SPLICED TRANSCRIPT OF EQUINE INTERLEUKIN-1-BETA", Gene, 177(1-2), 1996, pp. 11-16

Abstract

Using lipopolysaccharide (LPS)-stimulated equine peripheral blood mononuclear cell (PBMC) cDNA as a template, we performed polymerase chainreaction (PCR) amplification with equine interleukin-1 beta (IL-1 beta) specific primers. Electrophoresis of the PCR product on agarose gelrevealed an additional smaller fragment that hybridized with an equine IL-1 beta cDNA probe. Sequencing of this fragment demonstrated that it was shorter than normal equine IL-1 beta cDNA by 162 nucleotides, which corresponded to exon 5 of the human and murine IL-1 beta genes. The deletion of 162 nucleotides did not result in a frame shift but spliced out the putative exon 5 of the IL-1 beta gene which includes the cleavage site for the IL-1 beta converting enzyme (ICE) in human and murine IL-1 beta. Expression of the alternatively spliced IL-1 beta transcript in PBMC was also detected after stimulation with other compounds. These results clearly indicate the existence of an alternatively spliced IL-1 beta transcript in equine PBMC.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/11/20 alle ore 20:35:38