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Titolo:
EFFECTS OF NITRIC-OXIDE ON CHONDROCYTE MIGRATION, ADHESION, AND CYTOSKELETAL ASSEMBLY
Autore:
FRENKEL SR; CLANCY RM; RICCI JL; DICESARE PE; REDISKE JJ; ABRAMSON SB;
Indirizzi:
HOSP JOINT DIS & MED CTR,301 E 17TH ST NEW YORK NY 10003 NYU,MED CTR NEW YORK NY 10016 CIBA PHARMACEUT SUMMIT NJ 00000
Titolo Testata:
Arthritis and rheumatism
fascicolo: 11, volume: 39, anno: 1996,
pagine: 1905 - 1912
SICI:
0004-3591(1996)39:11<1905:EONOCM>2.0.ZU;2-Q
Fonte:
ISI
Lingua:
ENG
Soggetto:
ARTICULAR-CARTILAGE REPAIR; ALLOGRAFT CHONDROCYTES; PROTEOGLYCAN SYNTHESIS; HUMAN NEUTROPHILS; SYNTHASE; DEFECTS; EXPRESSION; INDUCTION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
50
Recensione:
Indirizzi per estratti:
Citazione:
S.R. Frenkel et al., "EFFECTS OF NITRIC-OXIDE ON CHONDROCYTE MIGRATION, ADHESION, AND CYTOSKELETAL ASSEMBLY", Arthritis and rheumatism, 39(11), 1996, pp. 1905-1912

Abstract

Objective. The migration of cells of chondrocyte lineage is believed to play a role in cartilage growth and repair, The present study examined 1) whether chondrocytes are capable of migration in vitro; and 2) the effects of nitric oxide (NO) on chondrocyte migration, adhesion, and cytoskeletal assembly. Methods. Chondrocyte migration was evaluatedby 2 assays: 1) ''centrifugal'' migration within a 3-dimensional collagen matrix (dot culture); and 2) directed migration under agarose in response to bone morphogenetic protein, To assess the effects of NO, chondrocytes were treated with either exogenous NO (S-nitrosoglutathione [SNO-GSH]) or a mixture of cytokines known to induce endogenous NO production, The effects of NO on chondrocyte adhesion to fibronectin-coated surfaces, as well as on actin polymerization (determined by indirect immunofluorescence), were also examined. Results. The capacity of chondrocytes to migrate was demonstrated both by the dot culture and by agarose methods, Both SNO-GSH and endogenous NO induced by cytokinesinhibited this migration, Exposure to NO also inhibited attachment ofchondrocytes to fibronectin and disrupted assembly of actin filaments, These effects of SNO-GSH and cytokine-induced NO production were reversed in the presence of hemoglobin and the NO synthase inhibitor N-G-monomethyl arginine, respectively. Conclusion. NO interferes with chondrocyte migration and attachment to fibronectin, an extracellular matrix protein, probably via effects on the actin cytoskeleton, These effects of NO may result in impairment of cartilage repair, by interferingwith the extracellular matrix regulation of chondrocyte function.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 11/07/20 alle ore 13:37:45