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Titolo:
AUTOIMMUNITY TO RNA-POLYMERASE-II IS FOCUSED AT THE CARBOXYL-TERMINALDOMAIN OF THE LARGE SUBUNIT
Autore:
HIRAKATA M; KANUNGO J; SUWA A; TAKEDA Y; CRAFT J; HARDIN JA;
Indirizzi:
MED COLL GEORGIA,SCH MED,DEPT INTERNAL MED,BI W548,1120 15TH ST AUGUSTA GA 30912 MED COLL GEORGIA,SCH MED,DEPT INTERNAL MED AUGUSTA GA 30912 KEIO UNIV,SCH MED TOKYO JAPAN MED COLL GEORGIA AUGUSTA GA 30912 YALE UNIV,SCH MED NEW HAVEN CT 00000
Titolo Testata:
Arthritis and rheumatism
fascicolo: 11, volume: 39, anno: 1996,
pagine: 1886 - 1891
SICI:
0004-3591(1996)39:11<1886:ATRIFA>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
SYSTEMIC LUPUS-ERYTHEMATOSUS; SMALL NUCLEAR; MONOCLONAL-ANTIBODIES; EXTENSIVE HOMOLOGY; AUTO-ANTIGEN; AUTOANTIBODIES; SERA; PROTEINS; POLYPEPTIDES; PURIFICATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
36
Recensione:
Indirizzi per estratti:
Citazione:
M. Hirakata et al., "AUTOIMMUNITY TO RNA-POLYMERASE-II IS FOCUSED AT THE CARBOXYL-TERMINALDOMAIN OF THE LARGE SUBUNIT", Arthritis and rheumatism, 39(11), 1996, pp. 1886-1891

Abstract

Objective. Previous studies have demonstrated antibodies to the large(220 kd) polypeptide subunit of RNA polymerase II (Pol II) in sera from certain patients with scleroderma. In the present study, we sought to identify the autoantigenic region on this polypeptide. Methods. A recombinant fusion protein, corresponding to the 52-heptapeptide repeatfound in the carboxyl terminal domain (CTD) of the large Pol II subunit, was used to identify 15 patient sera that contained autoantibodies, Synthetic peptides CTD7 (representing a single heptapeptide) and CTD18 (representing 2 1/2 heptapeptide repeats) mere used in a competitive inhibition assay to define the specificity of these sera and the importance of the CTD as an autoantigen. Results. All 15 sera immunoprecipitated the Pol II subunit from radiolabeled cell extracts, and 11 of them bound the CTD fusion protein in immunoblots, Immunoprecipitation of Pol II was completely inhibited by CTD18 in 5 sera and partially inhibited in 4 additional sera. Conclusion. These results indicate that the CTD heptapeptide repeat is a focal point for autoimmune responses in scleroderma. It is likely that the repetitive sequence and high content of charged residues of this structure contribute to its role as an autoantigen.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/12/20 alle ore 18:08:47