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Titolo:
ACTIVATION OF ACTIN-CLEAVABLE INTERLEUKIN 1-BETA-CONVERTING ENZYME (ICE) FAMILY PROTEASE CPP-32 DURING CHEMOTHERAPEUTIC AGENT-INDUCED APOPTOSIS IN OVARIAN-CARCINOMA CELLS
Autore:
CHEN ZH; NAITO M; MASHIMA T; TSURUO T;
Indirizzi:
UNIV TOKYO,INST MOL & CELLULAR BIOSCI,LAB BIOMED RES,BUNKYO KU,1-1-1 YAYOI TOKYO 113 JAPAN UNIV TOKYO,INST MOL & CELLULAR BIOSCI,LAB BIOMED RES,BUNKYO KU TOKYO 113 JAPAN JAPANESE FDN CANC RES,CTR CANC CHEMOTHERAPY,TOSHIMA KU TOKYO 170 JAPAN
Titolo Testata:
Cancer research
fascicolo: 22, volume: 56, anno: 1996,
pagine: 5224 - 5229
SICI:
0008-5472(1996)56:22<5224:AOAI1E>2.0.ZU;2-U
Fonte:
ISI
Lingua:
ENG
Soggetto:
T-LYMPHOMA CELLS; DEATH GENE CED-3; INTERLEUKIN-1-BETA-CONVERTING ENZYME; POLY(ADP-RIBOSE) POLYMERASE; ENCODES; SUPPRESSION; INDUCTION; ADHESION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
30
Recensione:
Indirizzi per estratti:
Citazione:
Z.H. Chen et al., "ACTIVATION OF ACTIN-CLEAVABLE INTERLEUKIN 1-BETA-CONVERTING ENZYME (ICE) FAMILY PROTEASE CPP-32 DURING CHEMOTHERAPEUTIC AGENT-INDUCED APOPTOSIS IN OVARIAN-CARCINOMA CELLS", Cancer research, 56(22), 1996, pp. 5224-5229

Abstract

We have previously reported that actin cleavage activity (ACA) by interleukin 1 beta-converting enzyme (ICE) family protease was elevated during anticancer drug-induced apoptosis in human leukemia U937 cells. In this study, the involvement of ACA in the drug-induced apoptosis insolid tumor cells was investigated. Human ovarian carcinoma OVCAR-3 cells undergo apoptotic cell death when cells are treated with chemotherapeutic agents such as cisplatin and etoposide. The induction of the actin cleavage activity accompanied the development of apoptosis. ICE/ced-3 family protease inhibitors such as Z-VAD-CH2DCB and Z-EVD-CH2DCBat 100 mu g/ml prevented both the emergence of ACA and the morphological change, characteristics of apoptosis, in cisplatin-treated OVCAR-3cells. The ACA in apoptotic OVCAR-3 cell lysate was greatly adsorbed by antibody against CPP-32, an ICE family protease. Furthermore, the immunoprecipitated CPP-32 from OVCAR-3 lysate could cleave actin to generate a 15-kDa fragment, as did the apoptotic OVCAR-3 cell lysate, indicating that CPP-32 is a major protease responsible for the ACA. The activation of CPP-32 in the drug-treated cell lysate was verified with Western blot analysis. Our present results indicate that CPP-32, an actin cleavage ICE/ced-3 family protease, could be a common mediator involved in the process of chemotherapy-induced apoptosis of cancer cells.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 07/07/20 alle ore 21:33:18