Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
TIME-VARIANT ANALYSIS OF ORGANELLE AND VESICLE MOVEMENT DURING PHAGOCYTOSIS IN PARAMECIUM-PRIMAURELIA BY MEANS OF FLUORESCENCE CONFOCAL LASER-SCANNING MICROSCOPY
Autore:
RAMOINO P; BELTRAME F; DIASPRO A; FATO M;
Indirizzi:
UNIV GENOA,IST ZOOL,VIA BALBI 5 I-16126 GENOA ITALY UNIV GENOA,DEPT COMMUN COMP & SYST SCI I-16145 GENOA ITALY UNIV GENOA,DEPT PHYS I-16146 GENOA ITALY
Titolo Testata:
Microscopy research and technique
fascicolo: 5, volume: 35, anno: 1996,
pagine: 377 - 384
SICI:
1059-910X(1996)35:5<377:TAOOAV>2.0.ZU;2-F
Fonte:
ISI
Lingua:
ENG
Soggetto:
PHAGOSOME MEMBRANE; DIGESTIVE VACUOLES; ACID-PHOSPHATASE; PLASMA-MEMBRANE; ENDOCYTOSIS; CAUDATUM; DIFFERENTIATION; TRANSPORT;
Keywords:
FOOD VACUOLES; LYSOSOMES; ORGANELLE MOVEMENT; MEMBRANE RECYCLING; IMAGE ANALYSIS; FLUORESCENCE MICROSCOPY; CONFOCAL MICROSCOPY; CILIATED PROTOZOA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
41
Recensione:
Indirizzi per estratti:
Citazione:
P. Ramoino et al., "TIME-VARIANT ANALYSIS OF ORGANELLE AND VESICLE MOVEMENT DURING PHAGOCYTOSIS IN PARAMECIUM-PRIMAURELIA BY MEANS OF FLUORESCENCE CONFOCAL LASER-SCANNING MICROSCOPY", Microscopy research and technique, 35(5), 1996, pp. 377-384

Abstract

Vital fluorescent dyes (FITC-albumin, Texas Red-albumin, and acridineorange) were used together with a confocal laser scanning optical microscope (CLSM) to display and analyze formation, movement, and fusion of vesicles during the phagocytosis of Paramecium primaurelia, in the x-y-z-t space. By immobilizing living cells pulsed with a food vacuolemarker at successive times after chasing in unlabeled medium, the intracellular movement of food vacuoles from their formation at the cytostome to their egestion at the cytoproct was visualized, and food vacuoles were selected in a specific digestion stage. Small pinocytic vesicles are shown to evaginate from the vacuoles and move in the cytoplasm. These vesicles are transported toward the cytopharynx where they enlarge the membrane of the nascent food vacuoles or fuse with stage II food vacuoles, when the vacuoles of stage II increase their size, changing from an acidic to an alkaline status. A multimodal analysis of confocal fluorescence images and the false-color technique were used to visualize vesicle movement vs. time. Starting from three images of the same cell at succeeding time points, a composite image was generated by associating with each originally acquired image a different color corresponding to each sampling point in time. The composite image shows that vesicles move away from the food vacuole in a scattered manner exhibiting changes in direction. (C) 1996 Wiley-Liss, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 21/09/20 alle ore 06:03:54