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Titolo:
EVIDENCE INDICATING THAT THE TM4, EL2, AND TM5 OF THE MELANOCORTIN-3 RECEPTOR DO NOT PARTICIPATE IN LIGAND-BINDING
Autore:
SCHIOTH HB; MUCENIECE R; SZARDENINGS M; PRUSIS P; WIKBERG JES;
Indirizzi:
BIOMED CTR,BOX 591 S-75124 UPPSALA SWEDEN UNIV UPPSALA,DEPT PHARMACEUT PHARMACOL UPPSALA SWEDEN LATVIAN ACAD SCI,INST ORGAN SYNTH,PHARMACOL LAB LV-226006 RIGA LATVIA
Titolo Testata:
Biochemical and biophysical research communications
fascicolo: 3, volume: 229, anno: 1996,
pagine: 687 - 692
SICI:
0006-291X(1996)229:3<687:EITTTE>2.0.ZU;2-J
Fonte:
ISI
Lingua:
ENG
Soggetto:
MOLECULAR-CLONING; BIOLOGICAL-ACTIVITY; ALPHA-MELANOTROPIN; HORMONE; MSH; EXPRESSION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
21
Recensione:
Indirizzi per estratti:
Citazione:
H.B. Schioth et al., "EVIDENCE INDICATING THAT THE TM4, EL2, AND TM5 OF THE MELANOCORTIN-3 RECEPTOR DO NOT PARTICIPATE IN LIGAND-BINDING", Biochemical and biophysical research communications, 229(3), 1996, pp. 687-692

Abstract

The TM4, EL2 and TM5 show low amino acid homology within the MC receptor family. Three mutants of the human MC3 receptor were created in order to investigate the participation of these regions in ligand binding. The TM4, EL2 and TM5 were separately changed by multiple mutagenesis so that their amino acid sequences became identical with the human MC1 receptor. The mutants were expressed in COS cells and they bound peptide ligands in the same fashion as the wild type MC3 receptor clone. Our results indicate that the amino acids that were mutated in the MC3 receptor do nor affect the binding of MSH peptides. The data providefurther evidence, that the mutated regions may not participate at allin ligand binding, as indicated by modelling experiments and homologycomparison. (C) 1996 Academic Press

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 07/07/20 alle ore 12:50:23