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Titolo:
A NEW PROTOPLAST CULTURE SYSTEM IN DAUCUS-CAROTA L AND ITS APPLICATIONS FOR MUTANT SELECTION AND TRANSFORMATION
Autore:
DIRKS R; SIDOROV V; TULMANS C;
Indirizzi:
NUNHEMS ZADEN BV,POB 4005 NL-6080 AA HAELEN NETHERLANDS INST CELL BIOL & GENET ENGN KIEV UKRAINE
Titolo Testata:
Theoretical and Applied Genetics
fascicolo: 5-6, volume: 93, anno: 1996,
pagine: 809 - 815
SICI:
0040-5752(1996)93:5-6<809:ANPCSI>2.0.ZU;2-N
Fonte:
ISI
Lingua:
ENG
Soggetto:
CARROT; FUSION; CALLUS;
Keywords:
PROTOPLASTS; MUTANT; STREPTOMYCIN; CHLOROPHYLL DEFICIENCY; TRANSFORMATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
23
Recensione:
Indirizzi per estratti:
Citazione:
R. Dirks et al., "A NEW PROTOPLAST CULTURE SYSTEM IN DAUCUS-CAROTA L AND ITS APPLICATIONS FOR MUTANT SELECTION AND TRANSFORMATION", Theoretical and Applied Genetics, 93(5-6), 1996, pp. 809-815

Abstract

Petiole protoplasts from in. vitro-grown carrot plants are a very good alternative to traditionally obtained protoplasts from suspension cultures. High plating and regeneration efficiencies were obtained in most of the breeding lines that were tested. The embedding of the protoplasts in alginate was crucial for initiating cell division and furtherdevelopment. Several streptomycin resistant and chlorophyll-deficientplant lines were selected for using the petiole protoplast system. Maternally inherited streptomycin resistance was demonstrated by sexual crosses. Protoplast fusion of several chlorophyll-deficient lines did not result in complementation, indicating the cytoplasmic nature of the mutations. Petiole protoplasts were used for direct transformation with plasmid DNA pNUN7 containing NPTII as a selectable marker. High transformation frequencies (up to 1%) were obtained after PEG treatment of the protoplasts. Kanamycin resistance was shown to be inherited as a single dominant nuclear trait.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 24/11/20 alle ore 21:54:42