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Titolo:
TCR GENE RECOMBINATION AND ALPHA-BETA-GAMMA-DELTA LINEAGE DIVERGENCE - PRODUCTIVE TCR-BETA REARRANGEMENT IS NEITHER EXCLUSIVE NOR PRECLUSIVE OF GAMMA-DELTA CELL-DEVELOPMENT
Autore:
BURTRUM DB; KIM S; DUDLEY EC; HAYDAY AC; PETRIE HT;
Indirizzi:
MEM SLOAN KETTERING CANC CTR,PROGRAM IMMUNOL,BOX 341,1275 YORK AVE NEW YORK NY 10021 MEM SLOAN KETTERING CANC CTR,PROGRAM IMMUNOL NEW YORK NY 10021 YALE UNIV,DEPT BIOL NEW HAVEN CT 06520 CORNELL UNIV,GRAD SCH MED SCI NEW YORK NY 10021
Titolo Testata:
The Journal of immunology
fascicolo: 10, volume: 157, anno: 1996,
pagine: 4293 - 4296
SICI:
0022-1767(1996)157:10<4293:TGRAAL>2.0.ZU;2-L
Fonte:
ISI
Lingua:
ENG
Soggetto:
T-CELLS; THYMOCYTE DEVELOPMENT; IMMATURE THYMOCYTES; RECEPTOR GENE; CHAIN GENES; EXPRESSION; THYMUS; CD4;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
23
Recensione:
Indirizzi per estratti:
Citazione:
D.B. Burtrum et al., "TCR GENE RECOMBINATION AND ALPHA-BETA-GAMMA-DELTA LINEAGE DIVERGENCE - PRODUCTIVE TCR-BETA REARRANGEMENT IS NEITHER EXCLUSIVE NOR PRECLUSIVE OF GAMMA-DELTA CELL-DEVELOPMENT", The Journal of immunology, 157(10), 1996, pp. 4293-4296

Abstract

Two types of T lymphocytes can be generated intrathymically, distinguishable by either TCR-gamma delta or -alpha beta surface expression, Regulation of the intrathymic divergence of these cells is unresolved, at least in part because thymically derived gamma delta cells have rarely been studied, We used quantitative Southern blotting together withPCR-based cloning/sequencing and restriction fragment length polymorphism to analyze TCR-alpha and -beta gene recombination in thymically derived gamma delta cells, We found that TCR-beta gene recombination isa frequent occurrence in thymic gamma delta cells, Furthermore, not only do complete (V-D-J) TCR-beta gene rearrangements occur in thymic gamma delta cells, but the frequency of in-frame rearrangements is greater than would be predicted based upon random occurrence, In contrast,we show that thymically derived gamma delta cells do not make detectable rearrangements of the TCR-alpha locus, These studies clearly demarcate a point for alpha beta vs gamma delta commitment in the thymus, after TCR-beta but before TCR-alpha gene recombination, Further, while our data support gamma delta lineage commitment as a consequence of successful TCR-gamma and -delta gene rearrangement, we do not find support for a competitive model of lineage commitment, since productive TCR-beta gene rearrangement does not necessarily relegate cells to the alpha beta lineage.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/09/20 alle ore 02:26:27