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Titolo:
IDENTIFICATION OF AMINO-COMPOUNDS SYNTHESIZED AND TRANSLOCATED IN SYMBIOTIC PARASPONIA
Autore:
BAKER A; DODD CD; PARSONS R;
Indirizzi:
UNIV DUNDEE,DEPT BIOL SCI DUNDEE DD1 4HN SCOTLAND UNIV DUNDEE,DEPT BIOL SCI DUNDEE DD1 4HN SCOTLAND
Titolo Testata:
Plant, cell and environment
fascicolo: 11, volume: 19, anno: 1996,
pagine: 1249 - 1260
SICI:
0140-7791(1996)19:11<1249:IOASAT>2.0.ZU;2-T
Fonte:
ISI
Lingua:
ENG
Soggetto:
NITROGEN-FIXATION; ARACHIS-HYPOGAEA; ACIDS; 4-METHYLENEGLUTAMINE; NODULES; PURIFICATION; RHIZOBIUM; GROWTH; LEAVES; PLANTS;
Keywords:
PARASPONIA; ULMACEAE; AMMONIA ASSIMILATION; GC-MS; GS/GOGAT; 4-METHYLENEGLUTAMINE; 4-METHYLGLUTAMATE; N-2 FIXATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
34
Recensione:
Indirizzi per estratti:
Citazione:
A. Baker et al., "IDENTIFICATION OF AMINO-COMPOUNDS SYNTHESIZED AND TRANSLOCATED IN SYMBIOTIC PARASPONIA", Plant, cell and environment, 19(11), 1996, pp. 1249-1260

Abstract

We investigated the synthesis and translocation of amino compounds inParasponia, a genus of the Ulmaceae that represents the only non-legumes known to form a root nodule symbiosis with rhizobia. In the xylem sap of P. andersonii we identified asparagine, aspartate, glutamine, glutamate and significant quantities of a non-protein amino acid, 4-methylglutamate (2-amino-4-methylpentanedioic acid). This identification was confirmed by two methods, capillary gas chromatography (GC) electron ionization (EI) mass spectrometry (MS) and reverse phase high pressure liquid chromatography (HPLC) analysis of derivatized compounds. Inleaf, root and nodule samples from P. andersonii and P. parviflora wealso identified the related compounds 4-methyleneglutamate and 4-methyleneglutamine. Using N-15(2) labelling and GC-MS analysis of root nodule extracts we followed N-2 fixation and ammonia assimilation in P. andersonii root nodules and observed label initially in glutamine and subsequently in glutamate, suggesting operation of the glutamine synthetase/glutamine:2-oxoglutarate aminotransferase (GS/GOGAT) pathway. Importantly, we observed the incorporation of significant quantities of N-15 into 4-methylglutamate in nodules, demonstrating the de novo synthesis of this non-protein amino acid and suggesting a role in the translocation of N in symbiotic Parasponia.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/11/20 alle ore 21:47:33