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Titolo:
INDUCIBLE SYNTHESIS OF THE MITOMYCIN-C RESISTANCE GENE-PRODUCT (MCRA)FROM STREPTOMYCES-LAVENDULAE
Autore:
AUGUST PR; RAHN JA; FLICKINGER MC; SHERMAN DH;
Indirizzi:
UNIV MINNESOTA,DEPT MICROBIOL ST PAUL MN 55108 UNIV MINNESOTA,DEPT MICROBIOL ST PAUL MN 55108 UNIV MINNESOTA,DEPT BIOCHEM ST PAUL MN 55108 UNIV MINNESOTA,BIOL PROC TECHNOL INST,GORTNER LAB 240 ST PAUL MN 55108
Titolo Testata:
Gene
fascicolo: 1-2, volume: 175, anno: 1996,
pagine: 261 - 267
SICI:
0378-1119(1996)175:1-2<261:ISOTMR>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
THIOSTREPTON-INDUCED GENE; DNA DAMAGE; EXPRESSION; LIVIDANS; CLONING;
Keywords:
DRUG-INDUCIBLE PRODUCTION; MITOMYCINS; STREPTOMYCES LIVIDANS; CELLULAR SELF-PROTECTION; ENZYME-LINKED IMMUNOSORBENT ASSAY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
22
Recensione:
Indirizzi per estratti:
Citazione:
P.R. August et al., "INDUCIBLE SYNTHESIS OF THE MITOMYCIN-C RESISTANCE GENE-PRODUCT (MCRA)FROM STREPTOMYCES-LAVENDULAE", Gene, 175(1-2), 1996, pp. 261-267

Abstract

The mcr locus from Streptomyces lavendulae confers high level resistance (> 100 mu g/ml) to mitomycin C (MC) and related mitomycins when cloned into Streptomyces lividans. Production of the mcrA gene product (MCRA) was shown to be MC-inducible by identification of MCRA (M(r) of 54 kDa) using Western blot analysis and enzyme linked immunosorbent assay (ELISA). The magnitude of MCRA production was dependent on MC concentration, with primary induction starting at 0.1 mu g/ml and maximum induction at 10 mu g/ml of the drug. Different levels of MCRA production were observed when other mitomycin metabolites were used as inducers, and the level of induction related directly to aziridine ring substitution on the individual molecules. Moreover, inducible synthesis of the mcrA gene product was unique to this structural class since production of MCRA did not occur as a general response to DNA damaging agents. The time profile of intracellular MCRA synthesis correlated with MCproduction in S. lavendulae, suggesting coordinated regulation of MC resistance and biosynthetic genes.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/11/20 alle ore 02:16:15