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Titolo:
CLONING AND SEQUENCING OF A DEVELOPMENTALLY-REGULATED AVIAN MESSENGER-RNA CONTAINING THE LEA MOTIF FOUND IN PLANT SEED PROTEINS
Autore:
NIU S; ANTIN PB; MORKIN E;
Indirizzi:
UNIV ARIZONA,UNIV HEART CTR,6301 AHSC,1501 N CAMPBELL TUCSON AZ 85724 UNIV ARIZONA,UNIV HEART CTR TUCSON AZ 85724 UNIV ARIZONA,DEPT INTERNAL MED TUCSON AZ 85724 UNIV ARIZONA,DEPT PHYSIOL TUCSON AZ 85724 UNIV ARIZONA,DEPT PHARMACOL TUCSON AZ 85724
Titolo Testata:
Gene
fascicolo: 1-2, volume: 175, anno: 1996,
pagine: 187 - 191
SICI:
0378-1119(1996)175:1-2<187:CASOAD>2.0.ZU;2-4
Fonte:
ISI
Lingua:
ENG
Soggetto:
CHICK-EMBRYO; YOLK SAC; CELLS; 5-BROMODEOXYURIDINE; ERYTHROPOIESIS;
Keywords:
CHICK HEART CDNA LIBRARY; RECOMBINANT DNA; CHICK DEVELOPMENT;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
17
Recensione:
Indirizzi per estratti:
Citazione:
S. Niu et al., "CLONING AND SEQUENCING OF A DEVELOPMENTALLY-REGULATED AVIAN MESSENGER-RNA CONTAINING THE LEA MOTIF FOUND IN PLANT SEED PROTEINS", Gene, 175(1-2), 1996, pp. 187-191

Abstract

We report the cloning of a bromodeoxyuridine (BrdU)-sensitive transcript of 918 bp from an immortalized quail heart cell line containing anopen reading frame (ORF) of 215 amino acids (aa) (approximate to 23 kDa). Analysis of the secondary structure predicts two amphipathic alpha-helices with oppositely oriented amphipathic surfaces at the C-terminus of the protein. Each of the helices contains an LEA (late embryogenesis abundant) consensus sequence (A/TAEKAK/RETKD) which has been previously described only in a group of plant seed-specific proteins. Temporal and spatial distribution patterns of the transcript during chickembryo development were examined by whole-mount in situ hybridizationand Northern blot analysis. At H&H (Hamburger and Hamilton, 1951) stages 11-14, the message was expressed strongly in blood islands in the area opaca. At day 5, strong signals were found in the liver primordia, mesonephrons, and nephric duet. Frozen sections of whole mount-stained embryonic liver demonstrated that the message was restricted to developing blood cells. The expression pattern of this transcript suggests that its protein product may be involved in hematopoiesis during avian development.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 24/09/20 alle ore 08:21:10