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Titolo:
NOVEL ALTERNATIVE SPLICING PREDICTS A SECRETED EXTRACELLULAR ISOFORM OF THE HUMAN RECEPTOR-LIKE PROTEIN-TYROSINE-PHOSPHATASE LAR
Autore:
TABITI K; CUI L; CHHATWAL VJS; MOOCHHALA S; NGOI SS; PALLEN CJ;
Indirizzi:
NATL UNIV SINGAPORE,INST MOL & CELL BIOL,CELL REGULAT LAB,10 KENT RIDGE CRESCENT SINGAPORE 0511 SINGAPORE NATL UNIV SINGAPORE,INST MOL & CELL BIOL,CELL REGULAT LAB SINGAPORE 0511 SINGAPORE NATL UNIV SINGAPORE HOSP,DEPT SURG SINGAPORE 0511 SINGAPORE
Titolo Testata:
Gene
fascicolo: 1-2, volume: 175, anno: 1996,
pagine: 7 - 13
SICI:
0378-1119(1996)175:1-2<7:NASPAS>2.0.ZU;2-7
Fonte:
ISI
Lingua:
ENG
Soggetto:
LEUKOCYTE COMMON ANTIGEN; MESSENGER-RNA EXPRESSION; ENDOCRINE NEOPLASIA; COLORECTAL CANCERS; DCC GENE; DOMAINS; REGION; CHROMOSOME-1; ASSOCIATION;
Keywords:
RETAINED INTRON; TRUNCATED ISOFORM; FIBRONECTIN TYPE-III REPEAT; IMMUNOGLOBULIN REPEAT; COLON TUMORIGENESIS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
24
Recensione:
Indirizzi per estratti:
Citazione:
K. Tabiti et al., "NOVEL ALTERNATIVE SPLICING PREDICTS A SECRETED EXTRACELLULAR ISOFORM OF THE HUMAN RECEPTOR-LIKE PROTEIN-TYROSINE-PHOSPHATASE LAR", Gene, 175(1-2), 1996, pp. 7-13

Abstract

RT-PCR was used to examine the expression of LAR (encoding the leucocyte-common antigen-related protein tyrosine phosphatase) in normal human colon mucosa, and colon polyps and tumors. Although the LAR proteinwas not detected in the colon in a previous immunohistochemical study, amplification of a region of LAR between the most membrane proximal (eighth) fibronectin type-III (FN-III) repeat and the transmembrane domain demonstrated LAR expression in all samples, but showed no difference in expression within matched samples from each patient examined. An additional minor fragment amplified in all reactions was consistently observed in colon and various cell line samples using this and two other LAR-specific sets of primers. Cloning and sequencing of the fragment identified it as deriving from a novel alternatively spliced form of LAR containing a retained intron of 85 bp. This intron encodes an additional 13 amino acids followed by an in-frame stop codon, thus its retention is predicted to give rise to a secreted LAR extracellular region isoform(s). LAR transcripts containing the intron were detected by RNase protection assay of colon samples and were present in most human tissues examined by Northern analysis. A protein in colon tumor extract was recognized by antiserum raised to the intron-encoded sequence. Soluble isoforms of the LAR extracellular immunoglobulin (Ig)-like/FN-III repeat-containing region could have a biological function distinct from those isoforms localized at the cell surface and/or coupled tointracellular phosphatase activity.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/11/20 alle ore 02:01:28