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Titolo:
HIGHLY INFORMATIVE SINGLE-STRANDED CONFORMATION POLYMORPHISM (SSCP) OF SHORT TANDEM REPEATS IN DNA IDENTIFICATION
Autore:
LUCAS M; MUNOZ C; PINTADO E; SOLANO F;
Indirizzi:
FAC MED SEVILLE,DEPT BIOQUIM MED & BIOL MOL,MOL GENET LAB,AVDA SANCHEZ PIZJUAN 4 SEVILLE 41009 SPAIN
Titolo Testata:
Journal of forensic sciences
fascicolo: 1, volume: 42, anno: 1997,
pagine: 118 - 120
SICI:
0022-1198(1997)42:1<118:HISCP(>2.0.ZU;2-Z
Fonte:
ISI
Lingua:
ENG
Keywords:
FORENSIC SCIENCE; DNA TYPING; VARIABLE NUMBERS OF TANDEM REPEATS; CONFORMATIONAL POLYMORPHISMS; DISPUTED PATERNITY; MATCHING PROBABILITY; NONDENATURING PAGE; D1S80; CSF1PO; TPOX; THO1;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
5
Recensione:
Indirizzi per estratti:
Citazione:
M. Lucas et al., "HIGHLY INFORMATIVE SINGLE-STRANDED CONFORMATION POLYMORPHISM (SSCP) OF SHORT TANDEM REPEATS IN DNA IDENTIFICATION", Journal of forensic sciences, 42(1), 1997, pp. 118-120

Abstract

The analysis of short tandem repeats (STR) by PCR is a useful technique widely used in DNA identification. Some loci have alleles differingin the number of complete repeat units, whereas others are polymorphic with alleles differing even to the level of a single base. Minor differences in the sequence of STR can confuse the analysis when the PCR product are poorly resolved in denaturing PAGE. The study by SSCP of STR in loci CSF1PO, TPOX, and THO1 and the alleles of locus D1S80 is shown in a representative case of paternity dispute. The analysis of short tandem repeats (STR) by the conformation polymorphisms of single-stranded DNA (SSCP) clearly enhanced the degree of resolution. The high resolution of SSCP of the STR may be the result of the arrangement of very prominent secondary structure and conformation due to the primaryrepetitive sequence. The method may be of particular interest to distinguish STR with similar PAGE mobility under denaturing conditions andto distinguish tandem repeats of the same size but different sequence.

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Documento generato il 30/11/20 alle ore 20:07:18