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Titolo:
DECREASED H-3 THYMIDINE INCORPORATION BY HUMAN BLADDER EPITHELIAL-CELLS FOLLOWING EXPOSURE TO URINE FROM INTERSTITIAL CYSTITIS PATIENTS
Autore:
KEAY S; ZHANG CO; TRIFILLIS AL; HISE MK; HEBEL JR; JACOBS SC; WARREN JW;
Indirizzi:
UNIV MARYLAND,SCH MED,VET ADM MED CTR,ROOM 3B184,10 N GREENE ST BALTIMORE MD 21201 DEPT VET AFFAIRS MED CTR,RES SERV BALTIMORE MD 00000 DEPT VET AFFAIRS MED CTR,DEPT SURG,DIV UROL BALTIMORE MD 00000 DEPT VET AFFAIRS MED CTR,DEPT PATHOL BALTIMORE MD 00000 DEPT VET AFFAIRS MED CTR,DEPT MED,DIV INFECT DIS BALTIMORE MD 00000 DEPT VET AFFAIRS MED CTR,DEPT MED,DIV NEPHROL BALTIMORE MD 00000
Titolo Testata:
The Journal of urology
fascicolo: 6, volume: 156, anno: 1996,
pagine: 2073 - 2078
SICI:
0022-5347(1996)156:6<2073:DHTIBH>2.0.ZU;2-B
Fonte:
ISI
Lingua:
ENG
Keywords:
CYSTITIS; CHEMOTACTIC FACTORS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
20
Recensione:
Indirizzi per estratti:
Citazione:
S. Keay et al., "DECREASED H-3 THYMIDINE INCORPORATION BY HUMAN BLADDER EPITHELIAL-CELLS FOLLOWING EXPOSURE TO URINE FROM INTERSTITIAL CYSTITIS PATIENTS", The Journal of urology, 156(6), 1996, pp. 2073-2078

Abstract

Purpose: Interstitial cystitis (IC) is a chronic bladder disease of unknown etiology. We sought to determine whether a cytotoxin is presentin the urine of IC patients that could cause the epithelial damage seen in this disease. Materials and Methods: Evidence for a cytotoxin was sought using both a neutral red uptake viability assay in T24 bladder epithelial cells, and a H-3-thymidine incorporation assay in primarynormal adult bladder epithelial cells and FHS 738 Bl human fetal bladder cells. Results: The neutral red assay in T24 cells indicated the presence of a cytotoxin in 2 of 9 IC patient urine specimens. However, the more sensitive assay of cell proliferation (H-3-thymidine incorporation) in normal adult human bladder epithelial cells revealed antiproliferative activity in urine from 10 of 13 (77%) IC patients vs. 3 of 19 (16%) controls (two-way analysis of variance, p = .019). The antiproliferative activity of IC urine specimens was confirmed using FHS 738Bl human fetal bladder cells. The antiproliferative urinary substance(s) appeared to be a low molecular weight (<10,000 Dal, heat stable, trypsin-sensitive factor(s). Conclusions: Because a denuded or damaged bladder epithelium is a central finding in IC, it is possible that theantiproliferative protein(s) contributes to the pathogenesis of this disease.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/01/21 alle ore 04:31:41