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Titolo:
ALTERED EXPRESSION OF MYOSIN MESSENGER-RNA AND PROTEIN IN RAT SOLEUS AND TIBIALIS ANTERIOR FOLLOWING REINNERVATION
Autore:
HUEY KA; BODINE SC;
Indirizzi:
REGENERON PHARMACEUT INC,777 OLD SAW MILL RD TARRYTOWN NY 10591 UNIV CALIF SAN DIEGO,SCH MED,PROGRAM BIOMED SCI SAN DIEGO CA 92093 VET AFFAIRS MED CTR SAN DIEGO CA 92093
Titolo Testata:
American journal of physiology. Cell physiology
fascicolo: 6, volume: 40, anno: 1996,
pagine: 2016 - 2026
SICI:
0363-6143(1996)40:6<2016:AEOMMA>2.0.ZU;2-0
Fonte:
ISI
Lingua:
ENG
Soggetto:
HEAVY-CHAIN ISOFORMS; FAST-TWITCH MUSCLE; SKELETAL-MUSCLE; FREQUENCY STIMULATION; CROSS-REINNERVATION; MOTOR UNITS; SLOW; FIBER; TRANSFORMATION; HYBRIDIZATION;
Keywords:
RIBONUCLEASE PROTECTION ASSAY; MYOSIN HEAVY CHAINS; SCIATIC NERVE INJURY; ADAPTATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
36
Recensione:
Indirizzi per estratti:
Citazione:
K.A. Huey e S.C. Bodine, "ALTERED EXPRESSION OF MYOSIN MESSENGER-RNA AND PROTEIN IN RAT SOLEUS AND TIBIALIS ANTERIOR FOLLOWING REINNERVATION", American journal of physiology. Cell physiology, 40(6), 1996, pp. 2016-2026

Abstract

Myosin heavy chain (MHC) expression was studied in rat soleus and tibialis anterior (TA) at the mRNA and protein levels following reinnervation 8 and 32 wk after sciatic nerve injury. A sciatic nerve crush or transection injury was produced in the midthigh region of adult femaleSprague-Dasyley rats. A ribonuclease protection assay was developed to measure four of the adult MHCs (I, IIa, IIx, IIb) in a single sample. MHC mRNA and protein were measured and compared in the same muscles. Eight and thirty-two weeks after a crush injury, the MHC mRNA profiles were similar to control with the exception of soleus MHC IIa and TA MHC IIb, which were significantly less than control at both time points. In contrast, reinnervation of the soleus following a sciatic nerve transection injury resulted in an MHC isoform shift characterized by increases in the relative amounts of fast myosin (IIa and IIx) and a decrease in slow myosin. As expected, significant changes first occurredat the mRNA level followed by changes in protein expression. Thirty-two weeks after transection injury and repair, the primary MHC mRNA isoform in the soleus was MHC IIx. Moreover, at 32 wk, MHC IIb mRNA was detected in 50% of the reinnervated soleus following a transection injury. Reinnervation of the TA following sciatic nerve transection led toreplacement of the MHC IIb isoform with MHC IIx.

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Documento generato il 28/11/20 alle ore 03:36:34