Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
THE 3'-UNTRANSLATED REGIONS OF CHLOROPLAST GENES IN CHLAMYDOMONAS-REINHARDTII DO NOT SERVE AS EFFICIENT TRANSCRIPTIONAL TERMINATORS
Autore:
ROTT R; DRAGER RG; STERN DB; SCHUSTER G;
Indirizzi:
CORNELL UNIV,BOYCE THOMPSON INST PLANT RES,TOWER RD ITHACA NY 14853 CORNELL UNIV,BOYCE THOMPSON INST PLANT RES ITHACA NY 14853 TECHNION ISRAEL INST TECHNOL,DEPT BIOL IL-32000 HAIFA ISRAEL
Titolo Testata:
MGG. Molecular & general genetics
fascicolo: 6, volume: 252, anno: 1996,
pagine: 676 - 683
SICI:
0026-8925(1996)252:6<676:T3ROCG>2.0.ZU;2-#
Fonte:
ISI
Lingua:
ENG
Soggetto:
MESSENGER-RNA; SPINACH CHLOROPLAST; MITOCHONDRIAL-DNA; ESCHERICHIA-COLI; INVERTED REPEATS; END MATURATION; PLASTID GENOME; PETD GENE; EXPRESSION; POLYMERASE;
Keywords:
CHLAMYDOMONAS; TRANSCRIPTION TERMINATION; CHLOROPLAST TRANSFORMATION; RNA PROCESSING;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
39
Recensione:
Indirizzi per estratti:
Citazione:
R. Rott et al., "THE 3'-UNTRANSLATED REGIONS OF CHLOROPLAST GENES IN CHLAMYDOMONAS-REINHARDTII DO NOT SERVE AS EFFICIENT TRANSCRIPTIONAL TERMINATORS", MGG. Molecular & general genetics, 252(6), 1996, pp. 676-683

Abstract

A general characteristic of the 3' untranslated regions of plastid mRNAs is an inverted repeat sequence that can fold into a stem-loop structure. These stem-loops are superficially similar to structures involved in prokaryotic transcription termination, but were found instead toserve as RNA 3' end processing signals in spinach chloroplasts, and in the atpB mRNA of Chlamydomonas reinhardtii chloroplasts. In order tocarry out a broad study of the efficiency of the untranslated sequences at the 3' ends of chloroplast genes in Chlamydomonas to function astranscription terminators, we performed in vivo run-on transcription experiments using Chlamydomonas chloroplast transformants in which different 3' ends were inserted into the chloroplast genome between a petD promoter and a reporter gene. The results showed that none of the 3'ends that were tested, in either sense or antisense orientation, prevented readthrough transcription, and thus were not highly efficient transcription terminators. Therefore, we suggest that most or all of the3' ends of mature mRNAs in Chlamydomonas chloroplasts are formed by 3' end processing of longer precursors.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/01/20 alle ore 01:11:55