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Titolo:
MINIMAL CHANGE-LIKE GLOMERULAR ALTERATIONS INDUCED BY A HUMAN PLASMA FACTOR
Autore:
CHEUNG PK; KLOK PA; BAKKER WW;
Indirizzi:
UNIV GRONINGEN,DEPT PATHOL,OOSTERSINGEL 63 NL-9713 EZ GRONINGEN NETHERLANDS
Titolo Testata:
Nephron
fascicolo: 3, volume: 74, anno: 1996,
pagine: 586 - 593
SICI:
0028-2766(1996)74:3<586:MCGAIB>2.0.ZU;2-P
Fonte:
ISI
Lingua:
ENG
Soggetto:
CHANGE NEPHROTIC SYNDROME; RAT-KIDNEY; ADENOSINE DIPHOSPHATASE; ANTITHROMBOTIC ACTIVITY; FILTRATION BARRIER; CHANGE NEPHROPATHY; BASEMENT-MEMBRANE; POLYANION GPA; ANIONIC SITES; LYMPHOCYTE-T;
Keywords:
MINIMAL CHANGE NEPHROTIC SYNDROME; PERMEABILITY FACTOR; SELECTIVE PERMEABILITY; GLOMERULAR ECTO-ATPASE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
46
Recensione:
Indirizzi per estratti:
Citazione:
P.K. Cheung et al., "MINIMAL CHANGE-LIKE GLOMERULAR ALTERATIONS INDUCED BY A HUMAN PLASMA FACTOR", Nephron, 74(3), 1996, pp. 586-593

Abstract

Circulating factors, including the plasma protease (100KF) described previously, have been suspected to play a role in the pathogenesis of minimal change disease (MCD) for several decades. This factor was ableto induce MCD-like alterations in kidney tissue in vitro, i.e. impairment of glomerular polyanion (GPA), as well as glomerular ecto-ATPase. We conducted permeability studies using alternate perfusion of the rat kidney ex vivo according to standard techniques. Either native 100KF(n = 7) or control factor (n = 7) perfusion, followed by perfusion with diluted rat serum was carried out, while urine samples were collected by ureter cannulation. Total urinary protein (by spectrophotometry)as well as IgG (by ELISA) and albumin (by rocket electrophoresis) were measured. Sections of perfused kidneys were stained (immuno-) histochemically for GPA and glomerular ecto-ATPase, and the stainability wasquantified using image analysis and expressed as arbitrary units. Theresults show significantly increased protein leakage after perfusion of 100KF versus control factor(150.0 +/- 48.9 vs. 33.2 +/- 7.7 mu g/min, p less than or equal to 0.01), while the IgG/albumin ratio has decreased(12.0 +/- 9.4 vs. 26.9 +/- 14.4%, p less than or equal to 0.01). Plasma protein leakage after 100KF perfusion is associated with a significant loss of GPA (57.3 +/- 27.5 vs. 98.4 +/- 12.0, p less than or equal to 0.01) and significant decrease of glomerular ecto-ATPase expression (28.7 +/- 11.5 vs. 79.5 +/- 15.0, p less than or equal to 0.001). The capability of 100KF to induce MCD-like glomerular lesions, in association with selectively increased permeability for plasma proteins,suggests that this human plasma constituent may be important in the pathogenesis of MCD.

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Documento generato il 04/12/20 alle ore 12:25:09