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Titolo:
DE-NOVO SYNTHESIS OF FRUCTANS FROM SUCROSE IN-VITRO BY A COMBINATION OF 2 PURIFIED ENZYMES (SUCROSE-SUCROSE 1-FRUCTOSYL TRANSFERASE AND FRUCTAN-FRUCTAN 1-FRUCTOSYL TRANSFERASE) FROM CHICORY ROOTS (CICHORIUM-INTYBUS L)
Autore:
VANDENENDE W; VANLAERE A;
Indirizzi:
KATHOLIEKE UNIV LEUVEN,INST BOT,DEV BIOL LAB,MERCIERLAAN 92 B-3001 HEVERLEE BELGIUM KATHOLIEKE UNIV LEUVEN,INST BOT,DEV BIOL LAB B-3001 HEVERLEE BELGIUM
Titolo Testata:
Planta
fascicolo: 3, volume: 200, anno: 1996,
pagine: 335 - 342
SICI:
0032-0935(1996)200:3<335:DSOFFS>2.0.ZU;2-C
Fonte:
ISI
Lingua:
ENG
Soggetto:
EXCISED LEAVES; INVERTASE; BIOSYNTHESIS; PURIFICATION; PATTERNS; WHEAT;
Keywords:
ASTERACEAE; CICHORIUM (FRUCTAN BIOSYNTHESIS); FRUCTAN- FRUCTAN 1-FRUCTOSYL TRANSFERASE; INULIN; SUCROSE- SUCROSE 1-FRUCTOSYL TRANSFERASE; SUCROSE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
21
Recensione:
Indirizzi per estratti:
Citazione:
W. Vandenende e A. Vanlaere, "DE-NOVO SYNTHESIS OF FRUCTANS FROM SUCROSE IN-VITRO BY A COMBINATION OF 2 PURIFIED ENZYMES (SUCROSE-SUCROSE 1-FRUCTOSYL TRANSFERASE AND FRUCTAN-FRUCTAN 1-FRUCTOSYL TRANSFERASE) FROM CHICORY ROOTS (CICHORIUM-INTYBUS L)", Planta, 200(3), 1996, pp. 335-342

Abstract

Although fructans occur widely in several plant families and they have been a subject of investigation for decennia, the mechanism of theirbiosynthesis is not completely elucidated. We succeeded in purifying a fructan: fructan 1-fructosyl transferase (1-FFT; EC 2.4.1.100) from chicory roots (Cichorium intybus L. var. foliosum cv. Flash). In combination with the purified chicory root sucrose: sucrose 1-fructosyl transferase (1-SST; EC 2.4.1.99), this enzyme synthesized a range of naturally occurring chicory fructans (inulins) from sucrose as the sole substrate. Starting from physiologically relevant sucrose concentrations, inulins up to a degree of polymerization (DP) of about 20 were synthesized in vitro after 96 h at 0 degrees C. Neither 1-SST, nor 1-FFT alone could mediate the observed fructan synthesis. Fructan synthesis invitro was compared starting from 50, 100 and 200mM sucrose, respectively. The initiation of (DP > 3)-fructan synthesis was found to be correlated with a certain ratio of 1-kestose to sucrose. The data presented now provide strong evidence to validate the 1-SST/1-FFT model for in-vivo fructan synthesis, at least in the Asteraceae.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/12/20 alle ore 19:20:30