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Titolo:
PUTATIVE HUMAN BLUE-LIGHT PHOTORECEPTORS HCRY1 AND HCRY2 ARE FLAVOPROTEINS
Autore:
HSU DS; ZHAO XD; ZHAO SY; KAZANTSEV A; WANG RP; TODO T; WEI YF; SANCAR A;
Indirizzi:
UNIV N CAROLINA,SCH MED,DEPT BIOCHEM & BIOPHYS CHAPEL HILL NC 27599 UNIV N CAROLINA,SCH MED,DEPT BIOCHEM & BIOPHYS CHAPEL HILL NC 27599 HUMAN GENOME SCI INC ROCKVILLE MD 20850 KYOTO UNIV,CTR RADIAT BIOL,SAKYO KU KYOTO 60601 JAPAN
Titolo Testata:
Biochemistry
fascicolo: 44, volume: 35, anno: 1996,
pagine: 13871 - 13877
SICI:
0006-2960(1996)35:44<13871:PHBPHA>2.0.ZU;2-T
Fonte:
ISI
Lingua:
ENG
Soggetto:
DNA PHOTOREACTIVATING ENZYME; GENE; PHOTOLYASE; SEQUENCE; IDENTIFICATION; EXPRESSION; PATTERNS; TIMELESS; PROTEIN; CLONING;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
44
Recensione:
Indirizzi per estratti:
Citazione:
D.S. Hsu et al., "PUTATIVE HUMAN BLUE-LIGHT PHOTORECEPTORS HCRY1 AND HCRY2 ARE FLAVOPROTEINS", Biochemistry, 35(44), 1996, pp. 13871-13877

Abstract

Recently, a human cDNA clone with high sequence homology to the photolyase/blue-light photoreceptor family was identified. The putative protein encoded by this gene exhibited a strikingly high (48% identity) degree of homology to the Drosophila melanogaster (6-4) photolyase [Todo et al. (1996) Science 272, 109-112]. We have now identified a secondhuman gene whose amino acid sequence displays 73% identity to the first one and have named the two genes CRY1 and CRY2, respectively. The corresponding proteins hCRY1 and hCRY2 were purified and characterized as maltose-binding fusion proteins. Similar to other members of the photolyase/blue-light photoreceptor family, both proteins were found to contain FAD and a pterin cofactor. Like the plant blue-light photoreceptors, both hCRY1 and hCRY2 lacked photolyase activity on the cyclobutane pyrimidine dimer and the (6-4) photoproduct. We conclude that these newly discovered members of the photolyase/photoreceptor family are not photolyases and instead may function as blue-light photoreceptors in humans.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 22/01/20 alle ore 06:45:01