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Titolo:
HOT-SPOTS OF CREATINE-KINASE LOCALIZATION IN BRAIN - CEREBELLUM, HIPPOCAMPUS AND CHOROID-PLEXUS
Autore:
KALDIS P; HEMMER W; ZANOLLA E; HOLTZMAN D; WALLIMANN T;
Indirizzi:
ETH HONGGERBERG,INST CELL BIOL CH-8093 ZURICH SWITZERLAND ETH HONGGERBERG,INST CELL BIOL CH-8093 ZURICH SWITZERLAND CHILDRENS HOSP,DEPT NEUROL BOSTON MA 00000 HARVARD UNIV,SCH MED BOSTON MA 00000
Titolo Testata:
Developmental neuroscience
fascicolo: 5-6, volume: 18, anno: 1996,
pagine: 542 - 554
SICI:
0378-5866(1996)18:5-6<542:HOCLIB>2.0.ZU;2-3
Fonte:
ISI
Lingua:
ENG
Soggetto:
CENTRAL-NERVOUS-SYSTEM; RAT-BRAIN; SARCOPLASMIC-RETICULUM; CHICKEN CEREBELLUM; ENERGY-METABOLISM; PURKINJE NEURONS; MOUSE-BRAIN; MUSCLE-TYPE; DIFFERENTIAL DISTRIBUTION; CARDIAC-MUSCLE;
Keywords:
BERGMANN GLIA; BRAIN ENERGY METABOLISM; CEREBELLUM; CREATINE KINASE; EPITHELIAL CELLS OF CHOROID PLEXUS; GRANULAR AND PYRAMIDAL CELLS OF HIPPOCAMPUS; IMMUNOFLUORESCENCE MICROSCOPY; PURKINJE NEURONS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
75
Recensione:
Indirizzi per estratti:
Citazione:
P. Kaldis et al., "HOT-SPOTS OF CREATINE-KINASE LOCALIZATION IN BRAIN - CEREBELLUM, HIPPOCAMPUS AND CHOROID-PLEXUS", Developmental neuroscience, 18(5-6), 1996, pp. 542-554

Abstract

Creatine kinase (CK) isoenzymes, with emphasis on the mitochondrial CK isoenzymes, were characterized and localized in chicken cerebellum. Chicken cerebellar extracts analyzed by two-dimensional gels, using antipeptide antibodies specific for sarcomeric muscle-type mitochondrialCK (Mi(b)-CK) and revealed the presence of a Mi(b)-CK variant in avian cerebellum, This CK isoform was localized by immunofluorescence staining exclusively in the Purkinje neurons. The co-expression of this Mi(b)-CK together with cytosolic muscle-type MM-CK, as observed in the same Purkinje neurons, may reflect the specific energy requirements associated with highly fluctuating Ca2+ levels (Ca2+ spiking) in these specialized neurons. Ubiquitous brain-type mitochondrial Mi(a)-CK was found together with cytosolic BB-CK mainly in the glomeruli structures of the cerebellar granular layer. BB-CK, but much less so Mi(a)-CK, however, was also very prominent in Bergmann glial cells of the molecularlayer. Thus, an isoenzyme-specific differential localization of the two mitochondrial MI-CK isoenzymes in the chicken cerebellum is demonstrated. Other hot spots of CK localization were the granule and pyramidal cells of the hippocampus in rat. There, a developmental stage-dependent immunofluorescence staining, especially with antibodies against Mi(a)-CK, was noted. Epithelial cells of the choroid plexus were also highly enriched in CK, The possible implications of a CK/PCr circuit atthese various cellular locations of the brain are discussed with respect to normal brain physiology and pathology.

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Documento generato il 27/01/20 alle ore 14:41:59