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Titolo:
COMPARATIVE ANTAGONISM OF KAINATE-ACTIVATED KAINATE AND AMPA RECEPTORS IN HIPPOCAMPAL-NEURONS
Autore:
PATERNAIN AV; VICENTE A; NIELSEN EO; LERMA J;
Indirizzi:
CSIC,INST CAJAL,DEPT NEURAL PLAST,AV DOCTOR ARCE 37 E-28002 MADRID SPAIN CSIC,INST CAJAL,DEPT NEURAL PLAST E-28002 MADRID SPAIN NEUROSEARCH AS DK-2600 GLOSTRUP DENMARK
Titolo Testata:
European journal of neuroscience
fascicolo: 10, volume: 8, anno: 1996,
pagine: 2129 - 2136
SICI:
0953-816X(1996)8:10<2129:CAOKKA>2.0.ZU;2-3
Fonte:
ISI
Lingua:
ENG
Soggetto:
AMINO-ACID RECEPTORS; NMDA GLUTAMATE RECEPTOR; CENTRAL NERVOUS-SYSTEM; CONCANAVALIN-A; HIGH-AFFINITY; RAT-BRAIN; DESENSITIZATION; CYCLOTHIAZIDE; SUBUNITS; EXPRESSION;
Keywords:
KAINATE-SELECTIVE RECEPTOR; NS102; NS394; CNQX; CYCLOTHIAZIDE; IMMUNOCYTOCHEMISTRY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
38
Recensione:
Indirizzi per estratti:
Citazione:
A.V. Paternain et al., "COMPARATIVE ANTAGONISM OF KAINATE-ACTIVATED KAINATE AND AMPA RECEPTORS IN HIPPOCAMPAL-NEURONS", European journal of neuroscience, 8(10), 1996, pp. 2129-2136

Abstract

Native kainate receptors expressed by cultured hippocampal cells werestudied in the whole-cell configuration of the patch-clamp technique by using a fast perfusion system. About 80% of the neurons expressed kainate receptors independently of the time in culture (0-4 days), which coincided with the number of cells immunoreactive for a monoclonal antibody against the GluR5/6/7 subunits. Three types of cells were considered: neurons in which the rapid application of kainate induced a rapidly desensitizing current, cells in which kainate induced a more slowly rising, non-desensitizing, response and those in which a mixture of both responses was apparent. Steady responses induced by 300 mu M kainate were inhibited by 6-cyano-7-nitroquinoxaline-2,3-dione (CNQX) ina dose-dependent manner (IC50 = 0.92 mu M). CNQX was less potent in blocking transient kainate-induced responses (IC50 = 6.1 mu M). Responses to kainate, whether steady or transient, were also inhibited by NS102, showing poor selectivity for the transient response (IC50 = 4.1 and 2.2 mu M respectively). The new alpha-amino-3-hydroxy-5-methyl-4-isoxazole (AMPA) receptor antagonist NS394 was very potent in inhibiting steady kainate-induced currents (IC50 = 0.45 mu M), but was even more effective in preventing peak responses (IC50 = 0.13 mu M). In contrast, cyclothiazide did not affect transient kainate-induced responses butdid potentiate current induced by activation of AMPA receptors by AMPA or kainate. These results demonstrate the lack of complete selectivity amongst some available competitive antagonists for AMPA and kainatereceptors, and indicate that kainate receptors expressed by hippocampal cells lack the cyclothiazide modulatory site present at AMPA receptors. In addition, the present data support the idea that low-affinity kainate binding sites in the brain correspond to receptor channels selectively activated by kainate.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 11/07/20 alle ore 11:02:27