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Titolo:
LIPOPOLYSACCHARIDE ISOLATED FROM PORPHYROMONAS-GINGIVALIS GROWN IN HEMIN-LIMITED CHEMOSTAT CONDITIONS HAS A REDUCED CAPACITY FOR HUMAN NEUTROPHIL PRIMING
Autore:
CHAMPAGNE CM; HOLT SC; VANDYKE TE; GORDON BJ; SHAPIRA L;
Indirizzi:
BOSTON UNIV,MED CTR,GOLDMAN SCH GRAD DENT,DEPT PERIODONTOL & ORAL BIOL,700 ALBANY ST W-201 BOSTON MA 02118 UNIV TEXAS,HLTH SCI CTR,DEPT PERIODONT SAN ANTONIO TX 78284 HEBREW UNIV JERUSALEM,HADASSAH FAC DENT MED,DEPT PERIODONT IL-91905 JERUSALEM ISRAEL
Titolo Testata:
Oral microbiology and immunology
fascicolo: 5, volume: 11, anno: 1996,
pagine: 319 - 325
SICI:
0902-0055(1996)11:5<319:LIFPGI>2.0.ZU;2-V
Fonte:
ISI
Lingua:
ENG
Soggetto:
ESCHERICHIA-COLI LIPOPOLYSACCHARIDE; IRON TRANSPORT PROTEINS; BACTEROIDES-GINGIVALIS; ENHANCED RELEASE; BACTERIAL LIPOPOLYSACCHARIDE; POLYMORPHONUCLEAR LEUKOCYTES; BINDING-PROTEIN; SUPEROXIDE; VIRULENCE; W50;
Keywords:
PORPHYROMONAS GINGIVALIS; LIPOPOLYSACCHARIDES; NEUTROPHIL; SUPEROXIDE; HEMIN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
47
Recensione:
Indirizzi per estratti:
Citazione:
C.M. Champagne et al., "LIPOPOLYSACCHARIDE ISOLATED FROM PORPHYROMONAS-GINGIVALIS GROWN IN HEMIN-LIMITED CHEMOSTAT CONDITIONS HAS A REDUCED CAPACITY FOR HUMAN NEUTROPHIL PRIMING", Oral microbiology and immunology, 11(5), 1996, pp. 319-325

Abstract

One way prokaryotes respond to environmental stresses is by modifyingselected outer membrane components. Iron, in the form of hemin, has been shown to be a significant regulator of Porphyromonas gingivalis growth and virulence and of the expression of outer membrane proteins and lipopolysaccharide. Since lipopolysaccharide has profound effects onhost immune cells, this study compared the effect of hemin-restrictedand hemin-normal P. gingivalis growth conditions on lipopolysaccharide priming of N-formylmethionyl-leucyl-phenylalanine-induced superoxidegeneration by human neutrophils. P. gingivalis was grown in a chemostat under normal (5 mu g hemin/ml) and hemin-restricted (0.08 mu g hemin/ml) conditions. Purified lipopolysaccharide from both P. gingivalis normal and hemin-limited environments increased N-formylmethionyl-leucyl-phenylalanine-induced superoxide release by neutrophils in a dose-dependent manner. Lipopolysaccharide isolated from the hemin-normal conditions was a significantly more potent neutrophil priming agent than the Lipopolysaccharide isolated from hemin-restricted conditions. Addition of normal human serum enhanced the priming effect of both lipopolysaccharide preparations; this effect, however, was more evident with the hemin-normal lipopolysaccharide. Further, this enhancing effect ofserum was partly reduced in the presence of antibodies raised againstthe serum Lipopolysaccharide-binding protein. The differences in the biological activity of the two lipopolysaccharide preparations could be associated with structural differences detected by sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis. These results indicate that hemin availability affects regulation of an aspect of the gingivalis virulence, lipopolysaccharide-human neutrophils priming. The reduced capacity for neutrophil priming by hemin-restricted Lipopolysaccharide appears to be related to lipopolysaccharide-neutrophil interactions and not to serum factors. Targeting bacterial cell-surface components involved in hemin transport might be effective therapy for P. gingivalis-associated periodontal diseases.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/11/20 alle ore 15:47:53