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Titolo:
SARCOPLASMIC RETICULAR CA2-HEART-FAILURE DUE TO MYOCARDIAL-INFARCTION( PUMP ATPASE ACTIVITY IN CONGESTIVE)
Autore:
AFZAL N; DHALLA NS;
Indirizzi:
ST BONIFACE GEN HOSP,RES CTR,INST CARDIOVASC SCI,351 TACHE AVE WINNIPEG MB R2H 2A6 CANADA ST BONIFACE GEN HOSP,RES CTR,INST CARDIOVASC SCI WINNIPEG MB R2H 2A6 CANADA
Titolo Testata:
Canadian journal of cardiology
fascicolo: 10, volume: 12, anno: 1996,
pagine: 1065 - 1073
SICI:
0828-282X(1996)12:10<1065:SRCDTM>2.0.ZU;2-P
Fonte:
ISI
Lingua:
ENG
Soggetto:
CALCIUM-TRANSPORT; VENTRICULAR PERFORMANCE; CONTRACTILE FAILURE; RATS; PHOSPHORYLATION; MUSCLE;
Keywords:
CONGESTIVE HEART FAILURE; SARCOPLASMIC RETICULAR CA2+ TRANSPORT; SARCOPLASMIC RETICULAR CA2+-STIMULATED ATPASE; SACROPLASMIC RETICULAR PHOSPHORYLATION; SARCOPLASMIC RETICULAR PHOSPHOLIPIDS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
40
Recensione:
Indirizzi per estratti:
Citazione:
N. Afzal e N.S. Dhalla, "SARCOPLASMIC RETICULAR CA2-HEART-FAILURE DUE TO MYOCARDIAL-INFARCTION( PUMP ATPASE ACTIVITY IN CONGESTIVE)", Canadian journal of cardiology, 12(10), 1996, pp. 1065-1073

Abstract

OBJECTIVE: Earlier studies have shown a depression in the sarcoplasmic reticular (SR) Ca2+ uptake and gene expression in Ca2+ pump ATPase protein in congestive heart failure subsequent to myocardial infarction. it is the objective of this study to understand further the mechanisms of depressed SR Ca2+ pump activity in the failing heart. METHODS: Heart failure in rats was induced by occluding the left coronary arteryfor 16 weeks and the viable left ventricle was processed for the isolation of SR membranes. Sham-operated animals were used as control. Thecharacteristics of SR Ca2+ pump ATPase in the presence of different concentrations of K+, Ca2+ and ATP were examined anal the purity of these membranes was monitored by determining the marker enzyme activities, In addition to measuring changes in cyclic adenosine monophosphate (cAMP) protein kinase and Ca2+-calmodulin induced phosphorylation, alterations in SR phospholipid composition as well as sulfhydryl (SH) group content were investigated. RESULTS: Ca2+-stimulated ATPase activity,unlike Mg2+-ATPase activity, was depressed in the left ventricular SRfrom failing hearts as compared to control. The decrease in Ca2+-stimulated ATPase activity was seen at different concentrations of Ca2+, K and ATP but no changes in the affinities of the enzyme for Ca2+ and ATP were evident. The SR Ca2+-stimulated ATPase activities in the presence of both cAMP-dependent protein kinase and Ca2+-calmodulin were markedly decreased in the failing hearts when compared to control preparations. Furthermore, the P-32 incorporation in the presence of cAMP-dependent protein kinase or Ca2+-calmodulin was also reduced in the experimental heart SR membranes. The phospholipid composition of the SR membranes from the failing heart was markedly altered. No changes in SH-group or the degree of cross contamination with other membranes. were apparent in the failing heart SR. CONCLUSIONS: These results suggest that abnormalities in membrane phospholipid composition and phosphorylation of the enzyme may partly explain the observed depression in SR Ca2+ pump ATPase activity in heart failure following myocardial infarction.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/12/20 alle ore 20:23:34