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Titolo:
HUMAN NEUTROPHIL GELATINASE AND ASSOCIATED LIPOCALIN IN ADULT AND LOCALIZED JUVENILE PERIODONTITIS
Autore:
WESTERLUND U; INGMAN T; LUKINMAA PL; SALO T; KJELDSEN L; BORREGAARD N; TJADERHANE L; KONTTINEN YT; SORSA T;
Indirizzi:
UNIV HELSINKI,INST DENT,DEPT PERIODONTOL,POB 41 FIN-00014 HELSINKI FINLAND UNIV HELSINKI,INST DENT,DEPT PERIODONTOL FIN-00014 HELSINKI FINLAND UNIV HELSINKI,INST DENT,DEPT ORAL PATHOL FIN-00014 HELSINKI FINLAND UNIV OULU,INST DENT,DEPT ORAL SURG OULU FINLAND RIGSHOSP,GRANULOCYTE RES LAB DK-2100 COPENHAGEN DENMARK UNIV HELSINKI,INST DENT,DEPT PEDODONT & ORTHODONT FIN-00014 HELSINKI FINLAND UNIV HELSINKI,DEPT ANAT FIN-00014 HELSINKI FINLAND UNIV HELSINKI,DEPT MED 4 FIN-00014 HELSINKI FINLAND UNIV OULU,INST DENT,DEPT CARIOL OULU FINLAND
Titolo Testata:
Journal of dental research
fascicolo: 8, volume: 75, anno: 1996,
pagine: 1553 - 1563
SICI:
0022-0345(1996)75:8<1553:HNGAAL>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
GINGIVAL CREVICULAR FLUID; POLYMORPHONUCLEAR LEUKOCYTE COLLAGENASE; MATRIX METALLOPROTEINASES; TISSUE DESTRUCTION; INTERSTITIAL COLLAGENASES; INFLAMMATORY MEDIATORS; CELLULAR SOURCE; IDENTIFICATION; INHIBITOR; DISEASES;
Keywords:
GINGIVA; PERIODONTITIS; MATRIX METALLOPROTEINASES; NEUTROPHIL GELATINASE-ASSOCIATED LIPOCALIN; IMMUNOHISTOCHEMISTRY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
46
Recensione:
Indirizzi per estratti:
Citazione:
U. Westerlund et al., "HUMAN NEUTROPHIL GELATINASE AND ASSOCIATED LIPOCALIN IN ADULT AND LOCALIZED JUVENILE PERIODONTITIS", Journal of dental research, 75(8), 1996, pp. 1553-1563

Abstract

In search of direct in vivo evidence of matrix metalloproteinases (MMPs) in periodontal tissue destruction, we studied the presence and localization of MMP-9 and neutrophil gelatinase-associated lipocalin (NGAL) in adult periodontitis (AP) and localized juvenile periodontitis (LJP) gingival tissue specimens by immunohistochemistry, and the activities of gelatinases by Western blot, enzymography, and activity measurements, using radioactive gelatin as substrate in gingival crevicular fluid (GCF) and saliva. In gingival tissue obtained from AP and LJP patients, polymorphonuclear leukocyte (PMN) 92-kDa MMP-9 and NGAL were seen in the connective tissue, but both the sulcular and the oral epithelia were consistently negative. Whereas PMNs located in the gingival blood vessels showed strictly cytoplasmic MMP-9 and NGAL immunoreactivities, in the case of PMN extravasation the staining reactions extendedextracellularly. Gelatinase activities consisting mainly of 92-kDa gelatinase were increased in AP GCF relative to LJP GCF and periodontally healthy control GCF. Western blot with specific anti-NGAL antibodiesrevealed the presence of 25-kDa NGAL and its high-molecular-weight forms in AP and LJP GCF and saliva and in culture medium of oral keratinocytes, but not in gingival fibroblast culture medium. We conclude that extravasated degranulating PMNs are the major source of MMP-9 and NGAL in periodontitis gingiva, GCF, and saliva.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 24/09/20 alle ore 05:19:41