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Titolo:
THE EXPRESSION OF STEROIDOGENIC ENZYME GENES IN HUMAN VASCULAR CELLS
Autore:
HATAKEYAMA H; MIYAMORI I; TAKEDA Y; YAMAMOTO H; MABUCHI H;
Indirizzi:
KANAZAWA UNIV,SCH MED,DEPT INTERNAL MED 2,13-1 TAKARAMACHI KANAZAWA ISHIKAWA 920 JAPAN KANAZAWA UNIV,SCH MED,DEPT INTERNAL MED 2 KANAZAWA ISHIKAWA 920 JAPAN KANAZAWA UNIV,SCH MED,DEPT BIOCHEM KANAZAWA ISHIKAWA 920 JAPAN
Titolo Testata:
Biochemistry and molecular biology international
fascicolo: 3, volume: 40, anno: 1996,
pagine: 639 - 645
SICI:
1039-9712(1996)40:3<639:TEOSEG>2.0.ZU;2-U
Fonte:
ISI
Lingua:
ENG
Soggetto:
BIOSYNTHESIS; ALDOSTERONE;
Keywords:
ALDOSTERONE; STEROIDOGENIC ENZYME GENES; VASCULAR CELLS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
14
Recensione:
Indirizzi per estratti:
Citazione:
H. Hatakeyama et al., "THE EXPRESSION OF STEROIDOGENIC ENZYME GENES IN HUMAN VASCULAR CELLS", Biochemistry and molecular biology international, 40(3), 1996, pp. 639-645

Abstract

Recently, we demonstrated that aldosterone is produced by human vascular cells, and that vascular aldosterone is linked to angiotensin II-induced hypertrophy of vascular smooth muscle cells. We therefore examined whether genes encoding steroidogenic enzymes responsible for aldosterone biosynthesis from cholesterol are expressed in vascular cells. Using polymerase chain reaction after reverse transcription, type I and II 3 beta-HSD (3 beta-hydroxysteroid dehydrogenase), P-450c21 (21-hydroxylase), and P-450c18 (18-hydroxylase/oxidase) genes were found to be expressed both in endothelial cells and smooth muscle cells cultivated from human pulmonary arteries. However, P-450scc (cholesterol sidechain cleavage enzyme) and P-45011 beta (11 beta-hydroxylase) mRNAs could not be detected. These findings suggest that the enzyme system responsible for aldosterone production in human vascular cells is different from that found in the adrenal carter and that vascular aldosterone may be synthesized from metabolic intermediates which originate fromthe circulation. Extra-adrenal 3 beta-HSD and steroid 21-hydroxylase occur in a wide variety of tissues. Thus, human vascular cells can retain the ability to produce aldosterone by expressing the P-450c18 gene.

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Documento generato il 01/12/20 alle ore 09:39:41