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Titolo:
LIGAND-INDUCED AUTOREGULATION OF IL-2 RECEPTOR-ALPHA CHAIN EXPRESSIONIN MURINE T-CELL LINES
Autore:
DEMAISON C; CHASTAGNER P; MOREAU JL; THEZE J;
Indirizzi:
INST PASTEUR,UNITE IMMUNOGENET CELLULAIRE,25 RUE DR ROUX F-75015 PARIS FRANCE INST PASTEUR,UNITE IMMUNOGENET CELLULAIRE F-75015 PARIS FRANCE
Titolo Testata:
International immunology
fascicolo: 10, volume: 8, anno: 1996,
pagine: 1521 - 1528
SICI:
0953-8178(1996)8:10<1521:LAOIRC>2.0.ZU;2-V
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN INTERLEUKIN-2 RECEPTOR; MONOCLONAL-ANTIBODY; HIGH-AFFINITY; GAMMA-CHAIN; BETA-CHAIN; CLONING; MOUSE; INDUCTION; PATHWAYS; COMPLEX;
Keywords:
ACTIVATION; CYTOKINE RESPONSIVENESS; IL-2; IL-2 RECEPTOR ALPHA; BETA AND GAMMA CHAINS; PROLIFERATION; T CELL;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
28
Recensione:
Indirizzi per estratti:
Citazione:
C. Demaison et al., "LIGAND-INDUCED AUTOREGULATION OF IL-2 RECEPTOR-ALPHA CHAIN EXPRESSIONIN MURINE T-CELL LINES", International immunology, 8(10), 1996, pp. 1521-1528

Abstract

The IL-2 receptor (IL-2R) is composed of three chains alpha, beta andgamma, In mice, contrary to the human system, we have previously demonstrated that the IL-2R beta gamma complex does not bind IL-2, Therefore, mouse IL-2 response is completely dependent on the expression of the IL-2R alpha gene product, T cell clones expressing mouse IL-2R betagamma and the human IL-2R alpha transgene have been studied, When cells are grown in IL-4, mouse IL-2R alpha is not expressed, However, exposure to IL-2 leads to the expression of the endogenous murine IL-2R alpha subunit, The T cell line expressing mouse IL-2R gamma and human IL-2R beta can grow in IL-2 but does not express endogenous murine IL-2R alpha. Transfection of these cells with the human IL-2R alpha gene restores the capacity to induce murine IL-2R alpha, This result demonstrates that IL-2-IL-2R alpha interactions are required for induction ofIL-2R alpha. The kinetics of induction and deinduction of murine IL-2R alpha have been studied using clone 18.III. From negative cells, expression of murine IL-2R alpha is a very slow phenomenon, From cells fully expressing IL-2R alpha, deinduction is a two-step process: after arapid decrease of IL-2R alpha the cells continue to express, for a long period of time, basal levels of murine IL-2R alpha, When cells expressing basal levels of IL-2R alpha are exposed to IL-2, induction of IL-2R alpha is a very rapid phenomenon, The autoregulatory loop formed by IL-2-IL-2R alpha therefore displays different levels of functioning.

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Documento generato il 28/01/20 alle ore 21:08:16