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Titolo:
AN IN-VITRO INVASION MODEL FOR HUMAN RENAL-CELL CARCINOMA CELL-LINES MIMICKING THEIR METASTATIC ABILITIES
Autore:
NAKAYAMA Y; NAITO S; RYUTO M; HATA Y; ONO M; SUEISHI K; KOMIYAMA S; ITOH H; KUWANO M;
Indirizzi:
UNIV OCCUPAT & ENVIRONM HLTH,DEPT SURG,YAHATA NISHI KU KITAKYUSHU FUKUOKA 807 JAPAN UNIV OCCUPAT & ENVIRONM HLTH,DEPT SURG 1 KITAKYUSHU FUKUOKA 807 JAPAN KYUSHU UNIV,SCH MED,DEPT BIOCHEM FUKUOKA 812 JAPAN KYUSHU UNIV,SCH MED,DEPT UROL FUKUOKA 812 JAPAN KYUSHU UNIV,SCH MED,DEPT PATHOL FUKUOKA 812 JAPAN KYUSHU UNIV,SCH MED,DEPT OTORHINOLARYNGOL FUKUOKA 812 JAPAN
Titolo Testata:
Clinical & experimental metastasis
fascicolo: 5, volume: 14, anno: 1996,
pagine: 466 - 474
SICI:
0262-0898(1996)14:5<466:AIIMFH>2.0.ZU;2-S
Fonte:
ISI
Lingua:
ENG
Soggetto:
MICROVASCULAR ENDOTHELIAL-CELLS; HEPARAN-SULFATE PROTEOGLYCAN; HEPATOCYTE GROWTH-FACTOR; MURINE TUMOR-CELLS; BASEMENT-MEMBRANE; PLASMINOGEN-ACTIVATOR; QUANTITATIVE ASSAY; CANCER METASTASIS; DIFFERENT ORGANS; NUDE-MICE;
Keywords:
CALF PULMONARY ENDOTHELIAL CELLS; HUMAN RENAL CELL CARCINOMA; INVASION AND METASTASIS; IN VITRO INVASION MODEL;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
49
Recensione:
Indirizzi per estratti:
Citazione:
Y. Nakayama et al., "AN IN-VITRO INVASION MODEL FOR HUMAN RENAL-CELL CARCINOMA CELL-LINES MIMICKING THEIR METASTATIC ABILITIES", Clinical & experimental metastasis, 14(5), 1996, pp. 466-474

Abstract

We developed a modified in vitro invasion assay system using monolayers of vascular endothelial cells, A type I collagen gel was formed in plastic dishes, and overlaid with type IV collagen, Calf pulmonary arterial endothelial (CPAE) felis were seeded onto these plates, and incubated until they reached confluence. Five human renal cell carcinoma cell lines with various metastatic potentials in vivo were then seeded on the monolayer;er CPAE cells, and their colony formation and invasion activities were examined for 9 days, At dag 4, the highly metastaticfell lines increased the number of colony foci on monolayer CPAE cells several fold higher than their poorly metastatic counterpart, The horizontal spreading patterns were also different between poorly and highly metastatic cell lines. On day 9, the number of carcinoma foci thatpenetrated the monolayer of CPAE cells and type IV collagen sheets into type I collagen gels in highly metastatic cell lines greatly increased as compared with that of poorly metastatic cell lines. Our in vitro invasion assay using monolayer CPAE cells would be useful to evaluate protease activities and colony formation during invasion.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 23/09/20 alle ore 21:20:26