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Titolo:
THE METABOLISM OF 22-5(-6) AND OF DOCOSAHEXAENOIC ACID [22-6(-3)] COMPARED IN RAT HEPATOCYTES
Autore:
RETTERSTOL K; WOLDSETH B; CHRISTOPHERSEN BO;
Indirizzi:
UNIV OSLO,RIKSHOSP,INST CLIN BIOCHEM N-0027 OSLO NORWAY
Titolo Testata:
Biochimica et biophysica acta, L. Lipids and lipid metabolism
fascicolo: 3, volume: 1303, anno: 1996,
pagine: 180 - 186
SICI:
0005-2760(1996)1303:3<180:TMO2AO>2.0.ZU;2-G
Fonte:
ISI
Lingua:
ENG
Soggetto:
PERFORMANCE LIQUID-CHROMATOGRAPHY; POLYUNSATURATED FATTY-ACIDS; ISOLATED LIVER-CELLS; PEROXISOMAL RETROCONVERSION; RHESUS-MONKEYS; N-3; DEFICIENCY; BRAIN; PHOSPHOLIPIDS; BIOSYNTHESIS;
Keywords:
LIPID; DOCOSAPENTAENOIC ACID; DOCOSAHEXAENOIC ACID; ESSENTIAL FATTY ACID DEFICIENCY; PHOSPHOLIPID; PHOSPHOLIPID MOLECULAR SPECIES; (RAT HEPATOCYTE);
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
31
Recensione:
Indirizzi per estratti:
Citazione:
K. Retterstol et al., "THE METABOLISM OF 22-5(-6) AND OF DOCOSAHEXAENOIC ACID [22-6(-3)] COMPARED IN RAT HEPATOCYTES", Biochimica et biophysica acta, L. Lipids and lipid metabolism, 1303(3), 1996, pp. 180-186

Abstract

Elevated levels of 22:5(-6), which is the elongated and desaturated product of arachidonic acid, is induced by selective n - 3 fatty acid deficiency, especially in brain cortex. Less elongation and desaturation of 20:4(-6) than of 20:5(-3) has been found in intact rat liver cells in previous studies and is probably the main reason why so little 22:5(-6) is found under adequate nutritional conditions, The present study compares the metabolism of 22:5(-6) with the metabolism of 22:6(-3), the main n - 3 fatty acid in mammals. Freshly isolated rat liver cells were incubated with [1-C-14]22:5(-6) and [1-C-14]22:6(-3). Oxidation and esterification in triacylglycerols, diacylglycerols and phospholipids were studied. The phospholipid classes were separated and the different molecular species identified. Rats with essential fatty acid deficiency were compared with control rats. 22:5(-6) was found to be a good substrate for membrane phospholipid biosynthesis and was conserved well in the phospholipid fraction of the rat liver cells for more than 3 h of incubation. More 22:5(-6) was esterified in the total phospholipid fraction acid less was incorporated in triacylglycerols than observed with 22:6(-3) in hepatocytes from control animals. This was notthe case in animals with essential fatty acid deficiency. 22:5(-6) was esterified to a greater extent in phosphatidylcholine than 22:6(-3) in control cells bur not in essential fatty acid deficiency cells. More 22:5(-6) was coupled with 18:0 in the sn - 1 position of the phospholipid molecular species than 22:6(-3) was in control cells.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 13/07/20 alle ore 10:24:06