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Titolo:
MECHANISMS OF IMPAIRED BETA-ADRENOCEPTOR-INDUCED AIRWAY RELAXATION BYINTERLEUKIN-1-BETA IN-VIVO IN THE RAT
Autore:
KOTO H; MAK JCW; HADDAD EB; XU WB; SALMON M; BARNES PJ; CHUNG KF;
Indirizzi:
UNIV LONDON IMPERIAL COLL SCI TECHNOL & MED,NATL HEART & LUNG INST,DOVEHOUSE ST LONDON SW3 6LY ENGLAND UNIV LONDON IMPERIAL COLL SCI TECHNOL & MED,NATL HEART & LUNG INST LONDON SW3 6LY ENGLAND
Titolo Testata:
The Journal of clinical investigation
fascicolo: 8, volume: 98, anno: 1996,
pagine: 1780 - 1787
SICI:
0021-9738(1996)98:8<1780:MOIBAR>2.0.ZU;2-Y
Fonte:
ISI
Lingua:
ENG
Soggetto:
ADRENERGIC RECEPTORS; MESSENGER-RNA; SMOOTH-MUSCLE; GUINEA-PIGS; INHIBITION; ASTHMA; ACCUMULATION; CYTOKINES; RESPONSES; INVITRO;
Keywords:
BETA(2)-ADRENERGIC RECEPTORS; CYCLIC AMP; ADENYLYL CYCLASE; G PROTEIN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
36
Recensione:
Indirizzi per estratti:
Citazione:
H. Koto et al., "MECHANISMS OF IMPAIRED BETA-ADRENOCEPTOR-INDUCED AIRWAY RELAXATION BYINTERLEUKIN-1-BETA IN-VIVO IN THE RAT", The Journal of clinical investigation, 98(8), 1996, pp. 1780-1787

Abstract

We studied the in vivo mechanism of beta-adrenergic receptor (beta-AR) hyporesponsiveness induced by intratracheal instillation of interleukin-1 beta (IL-1 beta, 500 U) in Brown-Norway rats, Tracheal and bronchial smooth muscle responses were measured under isometric conditions ex vivo, Contractile responses to electrical field stimulation and to carbachol were not altered, but maximal relaxation induced by isoproterenol (10(-6)-10(-5) M) was significantly reduced 24 h after IL-1 betatreatment in tracheal tissues and to a lesser extent, in the main bronchi, Radioligand binding using [I-125]iodocyanopindolol revealed a 32/-7% reduction in beta-ARs in lung tissues from IL-1 beta-treated rats, without any significant changes in beta(2)-AR mRNA level measured by Northern blot analysis. Autoradiographic studies also showed significant reduction in beta(2)-AR in the airways, Isoproterenol-stimulated cyclic AMP accumulation was reduced by IL-1 beta at 24 h in trachea and lung tissues. Pertussis toxin reversed this hyporesponsiveness to isoproterenol but not to forskolin in lung tissues, Western blot analysis revealed an IL-1 beta-induced increase in G(i) alpha protein expression, Thus, IL-1 beta induces an attenuation of beta-AR-induced airway relaxation through mechanisms involving a reduction in beta-ARs, an increase in G(i) alpha subunit, and a defect in adenylyl cyclase activity.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/12/20 alle ore 09:47:07