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Titolo:
ISOLATION AND CHARACTERIZATION OF ENDOTHELIAL-CELLS FROM DIFFERENT ORGANS OF FETAL PIGS
Autore:
PLENDL J; NEUMULLER C; VOLLMAR A; AUERBACH R; SINOWATZ F;
Indirizzi:
UNIV MUNICH,FAC VET MED,INST VET ANAT,VETERINARSTR 13 D-80539 MUNICH GERMANY UNIV MUNICH,FAC VET MED,INST PHARMACOL & TOXICOL D-80539 MUNICH GERMANY CTR DEV BIOL ZOOL RES MADISON WI 53706
Titolo Testata:
Anatomy and embryology
fascicolo: 5, volume: 194, anno: 1996,
pagine: 445 - 456
SICI:
0340-2061(1996)194:5<445:IACOEF>2.0.ZU;2-6
Fonte:
ISI
Lingua:
ENG
Soggetto:
WEIBEL-PALADE BODIES; GRIFFONIA SIMPLICIFOLIA-I; LOW-DENSITY LIPOPROTEIN; VONWILLEBRAND-FACTOR; BLOOD-VESSEL; MICROVASCULAR ENDOTHELIUM; LYCOPERSICON-ESCULENTUM; VASCULAR ENDOTHELIUM; HUMAN BRAIN; CULTURE;
Keywords:
ENDOTHELIUM; FETUS; PORCINE; IN VITRO; LECTINS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
67
Recensione:
Indirizzi per estratti:
Citazione:
J. Plendl et al., "ISOLATION AND CHARACTERIZATION OF ENDOTHELIAL-CELLS FROM DIFFERENT ORGANS OF FETAL PIGS", Anatomy and embryology, 194(5), 1996, pp. 445-456

Abstract

The purpose of this study was to isolate endothelial cells from different organs of porcine fetuses and to examine the binding of endothelial markers including lectins. Endothelial cells were isolated from theaorta, cerebral cortex, myocardium, ovary and testis. Binding of the antibodies to von Willebrand factor (vWF) and angiotensin converting enzyme (ACE), the presence of Weibel Palade bodies (WPB), uptake of acetylated low density lipoprotein (acLDL), and labelling with the lectins Bandeiraea simplicifolia agglutinin I(BS I), Peanut agglutinin (PNA), Dolichos biflorus agglutinin (DBA) and Ulex europaeus agglutinin I (UEA I) were examined. Cell preparations displayed cobblestone-like morphology with the exception of testicular endothelium, which formed arcuate structures. Endothelium isolated from the brain labelled more strongly than any other cell line with the lectin PNA, but it did not express ACE. In contrast to other cell preparations, myocardial endothelium showed very low binding of anti-vWF Ovarian endothelium was able toperform in vitro angiogenesis. Moreover, these endothelial cells possessed the largest number of WPB. Testicular endothelium displayed highest binding of vWF Endothelium isolated from the aorta, in contrast toall other endothelial cells, did not take up acLDL. These results demonstrate that organ- and tissue-specific heterogeneity is already expressed in fetal endothelium.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 03/12/20 alle ore 15:16:14