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Titolo:
RATIONAL MOLECULAR DESIGN AND GENETIC-ENGINEERING OF HERBICIDE-RESISTANT CROPS BY STRUCTURE MODELING AND SITE-DIRECTED MUTAGENESIS OF ACETOHYDROXYACID SYNTHASE
Autore:
OTT KH; KWAGH JG; STOCKTON GW; SIDOROV V; KAKEFUDA G;
Indirizzi:
AMER CYANAMID CO,AGR PROD RES DIV,POB 400 PRINCETON NJ 08543 AMER CYANAMID CO,AGR PROD RES DIV PRINCETON NJ 08543
Titolo Testata:
Journal of Molecular Biology
fascicolo: 2, volume: 263, anno: 1996,
pagine: 359 - 368
SICI:
0022-2836(1996)263:2<359:RMDAGO>2.0.ZU;2-O
Fonte:
ISI
Lingua:
ENG
Soggetto:
FLAVIN ADENINE-DINUCLEOTIDE; THALIANA VAR COLUMBIA; ACETOLACTATE SYNTHASE; SULFOMETURON METHYL; PYRUVATE OXIDASE; GENERAL-METHOD; BINDING-SITE; IMIDAZOLINONES; SEQUENCE; PROTEINS;
Keywords:
MOLECULAR ENGINEERING; GENE EXPRESSION; IMIDAZOLINONES; PROTEIN HOMOLOGY MODELING; INHIBITION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
27
Recensione:
Indirizzi per estratti:
Citazione:
K.H. Ott et al., "RATIONAL MOLECULAR DESIGN AND GENETIC-ENGINEERING OF HERBICIDE-RESISTANT CROPS BY STRUCTURE MODELING AND SITE-DIRECTED MUTAGENESIS OF ACETOHYDROXYACID SYNTHASE", Journal of Molecular Biology, 263(2), 1996, pp. 359-368

Abstract

Plants with specific resistance to a single class of herbicides have been genetically engineered by introduction of genes encoding rationally designed mutant acetohydroxyacid synthase (AHAS) enzymes. Suitable substitution mutations were identified from a three-dimensional model of an AHAS-inhibitor complex. The structural model was generated basedon homology to pyruvate oxidase and an imidazolinone inhibitor was positioned in the proposed binding site using structure-activity data for this class of herbicide. Biochemical analysis of the mutant proteinsexpressed in Escherichia coli enabled iterative optimization of the mutant genes. Expression of recombinant proteins in tobacco plants conferred resistance in vivo. The novel approach coupling molecular modeling and molecular biology has many advantages over traditional random mutagenesis and selection methods and will be crucial to the future development for environmentally safe and sustainable agricultural systems. (C) 1996 Academic Press Limited

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 24/11/20 alle ore 10:46:00