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Titolo:
EXPRESSION OF ACETYLCHOLINE-RECEPTOR GENES IN HUMAN THYMIC EPITHELIAL-CELLS - IMPLICATIONS FOR MYASTHENIA-GRAVIS
Autore:
WAKKACH A; GUYON T; BRUAND C; TZARTOS S; COHENKAMINSKY S; BERRIHAKNIN S;
Indirizzi:
HOP MARIE LANNELONGUE,CNRS URA 1159,133 AVE RESISTANCE F-92350 LE PLESSIS ROBINS FRANCE HELLENIC PASTEUR INST ATHENS GREECE
Titolo Testata:
The Journal of immunology
fascicolo: 8, volume: 157, anno: 1996,
pagine: 3752 - 3760
SICI:
0022-1767(1996)157:8<3752:EOAGIH>2.0.ZU;2-Y
Fonte:
ISI
Lingua:
ENG
Soggetto:
MAIN IMMUNOGENIC REGION; ALPHA-SUBUNIT VARIANTS; MONOCLONAL-ANTIBODIES; MESSENGER-RNA; RAT MYOTUBES; MUSCLE; THYMOMAS; PROTEIN; LYMPHOCYTES; FORSKOLIN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
52
Recensione:
Indirizzi per estratti:
Citazione:
A. Wakkach et al., "EXPRESSION OF ACETYLCHOLINE-RECEPTOR GENES IN HUMAN THYMIC EPITHELIAL-CELLS - IMPLICATIONS FOR MYASTHENIA-GRAVIS", The Journal of immunology, 157(8), 1996, pp. 3752-3760

Abstract

The intrathymic presence of the muscle acetylcholine receptor (AChR) is controversial, and the nature of the cell(s) expressing it is unclear, We thus analyzed the molecular expression of muscle AChR in human thymi, mRNA studies indicated that the two isoforms (P3A(+) and P3A(-)) of the alpha-subunit were present in thymic extracts and in culturedthymic epithelial cells (TEC), while expression in thymocytes was lowand not consistently detectable, The amount of mRNA coding for the alpha-subunit, evaluated by means of quantitative PCR, was about 20 times less in TEC than in muscle, and was similar in TEC from normal subjects and from patients with myasthenia gravis (MC), The beta- and epsilon-subunits present in adult AChR were also expressed in TEC (but not in thymocytes), while the embryonic subunit (gamma) was absent, In TECcultures, the AChR alpha- and epsilon-subunit mRNA levels were down-regulated by forskolin, as also observed in the TE671 rhabdomyosarcoma cell line, suggesting similar regulation of AChR subunits in thymus and muscle, Protein expression was evidenced on TEC (but not on thymocytes), by Western blotting as well as by immunofluorescence, thus demonstrating AChR expression on human thymic epithelial cells, There was nodifference in the expression of AChR between TEC from MC patients andcontrols, meaning that the expression of AChR subunits alone is not sufficient to explain the onset of MG.

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Documento generato il 30/11/20 alle ore 07:14:11