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Titolo:
IL-12-INDUCED ACTIVATION OF NK AND T-CELLS OCCURS IN THE ABSENCE OF IMMEDIATE-EARLY ACTIVATION GENE-EXPRESSION
Autore:
AZZONI L; KANAKARAJ P; ZATSEPINA O; PERUSSIA B;
Indirizzi:
JEFFERSON MED COLL,KIMMEL CANC INST,BLSB RM 750,233 S 10TH ST PHILADELPHIA PA 19107 JEFFERSON MED COLL,KIMMEL CANC INST PHILADELPHIA PA 19107
Titolo Testata:
The Journal of immunology
fascicolo: 8, volume: 157, anno: 1996,
pagine: 3235 - 3241
SICI:
0022-1767(1996)157:8<3235:IAONAT>2.0.ZU;2-H
Fonte:
ISI
Lingua:
ENG
Soggetto:
NATURAL-KILLER-CELLS; SIGNAL-TRANSDUCTION PATHWAYS; STIMULATORY FACTOR NKSF; RECEPTOR-BETA-CHAIN; JAK-3 JANUS KINASE; C-FOS PROMOTER; TYROSINE PHOSPHORYLATION; MOLECULAR MECHANISMS; GAMMA PRODUCTION; MESSENGER-RNA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
49
Recensione:
Indirizzi per estratti:
Citazione:
L. Azzoni et al., "IL-12-INDUCED ACTIVATION OF NK AND T-CELLS OCCURS IN THE ABSENCE OF IMMEDIATE-EARLY ACTIVATION GENE-EXPRESSION", The Journal of immunology, 157(8), 1996, pp. 3235-3241

Abstract

The responses of lymphocytes to IL-2 and IL-12, involving proliferation, differentiation, and cytokine production, are only partially overlapping, and may depend on induced differential expression of specific sets of genes. Using reverse-transcription PCR differential display, we isolated an mRNA species expressed in IL-2- but not IL-12-stimulatedNK cells. This was identified as the mRNA encoding the transcription factor egr-1, which is expressed with fast kinetics in T and NK cells upon IL-2, but not IL-12, stimulation. Analysis of the accumulation ofmRNA-encoding members of the AP-1 transcription factor family demonstrated that c-fos and junB are also expressed upon stimulation of NK and T cells with IL-2, but not IL-12, whereas expression of c-jun and junD is not modified by either cytokine. Accordingly, increased AP-1 DNA-binding activity and AP-l-dependent transcriptional activity were detected exclusively in IL-2-stimulated cells. Analysis of the expressionof genes reported to regulate cytokine-induced proliferation demonstrated that both IL-2 and IL-12 induce c-myc mRNA accumulation in NK andT cells, whereas only IL-2 induces bcl-2 expression. Our data providethe first demonstration that IL-12-mediated activation of T and NK cells does not involve expression of members of the immediate-early activation genes family (egr-1, c-fos, and junB), AP-1 transcriptional activity, or bcl-2 expression. This indicates that functional differencesobserved in IL-2- and IL-12-stimulated cells may depend, at least in part, on differential gene regulation.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 31/05/20 alle ore 13:30:43