Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
DIAGNOSTIC-VALUE OF WESTERN BLOTTING IN CARBOHYDRATE-DEFICIENT GLYCOPROTEIN SYNDROME
Autore:
SETA N; BARNIER A; HOCHEDEZ F; BESNARD MA; DURAND G;
Indirizzi:
HOP XAVIER BICHAT,LAB BIOCHIM A,46 RUE HUCHARD F-75877 PARIS 18 FRANCE HOP ROBERT DEBRE,SERV GASTROENTEROL PROF NAVARRO F-75019 PARIS FRANCE UFR PHARM,BIOCHIM LAB F-92296 CHATENAY MALABRY FRANCE
Titolo Testata:
Clinica chimica acta
fascicolo: 2, volume: 254, anno: 1996,
pagine: 131 - 140
SICI:
0009-8981(1996)254:2<131:DOWBIC>2.0.ZU;2-P
Fonte:
ISI
Lingua:
ENG
Soggetto:
SYNDROME TYPE-II; ALCOHOL-CONSUMPTION; SERUM TRANSFERRIN; ABNORMALITIES; VARIANT;
Keywords:
HAPTOGLOBIN; ALPHA-1-ACID GLYCOPROTEIN; ALPHA-1-ANTITRYPSIN; TRANSFERRIN; SDS-PAGE; CDG;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
31
Recensione:
Indirizzi per estratti:
Citazione:
N. Seta et al., "DIAGNOSTIC-VALUE OF WESTERN BLOTTING IN CARBOHYDRATE-DEFICIENT GLYCOPROTEIN SYNDROME", Clinica chimica acta, 254(2), 1996, pp. 131-140

Abstract

Carbohydrate-deficient glycoprotein syndrome (CDGS) is a newly recognized family of diseases characterized by the absence from the transferrin molecule of at least one glycan chain (type I) or an antenna of the glycan chain (type II). CDGS is currently diagnosed by studies of serum transferrin sialylation. We have developed an alternative Western blot-based method to detect serum transferrin species with reduced molecular masses due to altered glycosylation. Two additional bands are observed in type I CDGS, while a single lower band is observed in type II CDGS, relative to healthy subjects. N-glycanase treatment of serum from type I CDGS patients and normal subjects yields a single band of the same mass in the two cases, confirming that the glycan is the onlymoiety involved in the differential Western blot pattern. Similar results were found with serum al-acid glycoprotein, haptoglobin and alpha1-antitrypsin. Western-blot analysis of one or more serum glycoproteins permits the differential diagnosis of CDGS.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/11/20 alle ore 20:10:14