Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
IDENTIFICATION OF PLANT CYTOSKELETAL, CELL CYCLE-RELATED AND POLARITY-RELATED PROTEINS USING SCHIZOSACCHAROMYCES-POMBE
Autore:
XIA G; RAMACHANDRAN S; HONG Y; CHAN YS; SIMANIS V; CHUA NH;
Indirizzi:
ROCKEFELLER UNIV,PLANT MOL BIOL LAB,1230 YORK AVE NEW YORK NY 10021 ROCKEFELLER UNIV,PLANT MOL BIOL LAB NEW YORK NY 10021 NATL UNIV SINGAPORE,INST MOL AGROBIOL,LAB PLANT CELL BIOL SINGAPORE 118240 SINGAPORE SWISS INST EXPT CANC RES CH-1066 EPALINGES SWITZERLAND
Titolo Testata:
Plant journal
fascicolo: 4, volume: 10, anno: 1996,
pagine: 761 - 769
SICI:
0960-7412(1996)10:4<761:IOPCCC>2.0.ZU;2-B
Fonte:
ISI
Lingua:
ENG
Soggetto:
GTP-BINDING PROTEIN; FISSION YEAST; ARABIDOPSIS-THALIANA; MOLECULAR-CLONING; SACCHAROMYCES-CEREVISIAE; SEPTUM FORMATION; TUBULIN GENES; SHAPE CONTROL; SMALL GENOME; EXPRESSION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
47
Recensione:
Indirizzi per estratti:
Citazione:
G. Xia et al., "IDENTIFICATION OF PLANT CYTOSKELETAL, CELL CYCLE-RELATED AND POLARITY-RELATED PROTEINS USING SCHIZOSACCHAROMYCES-POMBE", Plant journal, 10(4), 1996, pp. 761-769

Abstract

The fission yeast Schizosaccharomyces pombe has been used to identifyArabidopsis thaliana proteins that may play a role in cell shape maintenance or cell cycle regulation. An Arabidopsis thaliana cDNA librarywas constructed in pREP5N vector under the control of the inducible nmt1 promoter and transformed into S. pombe. Expression of the A. thaliana sequences was induced and clones showing severe morphological changes were identified and analysed. Comparison of the sequences of the inserts with the sequence data bases revealed that several cDNAs encodeproteins known to play a role in function of the cytoskeleton, the cell cycle and establishment of cell polarity. These include alpha-1, alpha-2, alpha-6 and beta-6 tubulins, myosin heavy chain-like protein, ubiquitin conjugating enzymes UBC9 (E2), 26S protease subunits, Ran-binding protein, myb protein, PRL1 gene product and rho protein. Approximately 30% of the clones encode novel sequences. The results suggest that S. pombe phenotypic screening can be used to identify plant proteins involved in cell shape maintenance and regulation during cell cycle and development.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/04/20 alle ore 00:30:07