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Titolo:
RELEASE OF MALARIA CIRCUMSPOROZOITE PROTEIN INTO THE HOST-CELL CYTOPLASM AND INTERACTION WITH RIBOSOMES
Autore:
HUGEL FU; PRADEL G; FREVERT U;
Indirizzi:
NYU,MED CTR,DEPT MED & MOL PARASITOL NEW YORK NY 10010 NYU,MED CTR,DEPT MED & MOL PARASITOL NEW YORK NY 10010
Titolo Testata:
Molecular and biochemical parasitology
fascicolo: 2, volume: 81, anno: 1996,
pagine: 151 - 170
SICI:
0166-6851(1996)81:2<151:ROMCPI>2.0.ZU;2-V
Fonte:
ISI
Lingua:
ENG
Soggetto:
PLASMODIUM-BERGHEI SPOROZOITES; FALCIPARUM-INFECTED ERYTHROCYTES; FIBROBLAST GROWTH-FACTOR; INTERCELLULAR-ADHESION MOLECULE-1; LAMIN-B-RECEPTOR; HEPARAN-SULFATE; ENDOPLASMIC-RETICULUM; EXOERYTHROCYTIC SCHIZONTS; HUMAN HEPATOCYTES; CHRONIC HEPATITIS;
Keywords:
PLASMODIUM BERGHEI; CIRCUMSPOROZOITE PROTEIN; EXOERYTHROCYTIC FORM; PARASITOPHOROUS VACUOLE; ENDOPLASMIC RETICULUM; RIBOSOME;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
62
Recensione:
Indirizzi per estratti:
Citazione:
F.U. Hugel et al., "RELEASE OF MALARIA CIRCUMSPOROZOITE PROTEIN INTO THE HOST-CELL CYTOPLASM AND INTERACTION WITH RIBOSOMES", Molecular and biochemical parasitology, 81(2), 1996, pp. 151-170

Abstract

To date, the circumsporozoite (CS) protein has been implicated in guiding malaria sporozoites to the liver [Cerami et al., Cell 70, 1992, 1021-1033]. Here we show that shortly after invasion, P, berghei and P.yoelii sporozoites lie free in the invaded cell and release considerable amounts of CS protein into the cytoplasm. The intracytoplasmic deposition of CS protein begins during the attachment of the sporozoite to the host cell surface and reaches its peak during the first 4-6 h after invasion. Initially, the CS protein spreads over the entire cytoplasm of the infected cell where it interacts with cytosolic as well as endoplasmic reticulum-associated ribosomes. During the subsequent development of the parasites to exoerythrocytic forms, the CS protein binding becomes gradually restricted to ribosomes lining the outer membrane of the nuclear envelope of the host cell. The distribution pattern of the parasite-released CS protein in the host cell cytoplasm is independent of the permissiveness of the host cell for the development of the parasites to exoerythrocytic forms. It requires neither the host cell metabolism nor does it involve the endocytotic machinery. Recombinant P. falciparum CS protein interacts with RNAse-sensitive sites on endoplasmic reticulum-associated ribosomes as shown by microinjection and immunoelectron microscopy. The generalized interaction of the CS protein with host cell ribosomes suggests that the CS protein has an intracellular function during the hepatic phase in the life cycle of Plasmodium and may also explain the generation of a CD8(+) T cell response in the course of rodent malaria infections.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/09/20 alle ore 11:14:53