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Titolo:
NITRIC-OXIDE SYNTHASE INHIBITION NEGATES SEPTIC-INDUCED ALTERATIONS IN CYTOPLASMIC CALCIUM HOMEOSTASIS AND MEMBRANE DYNAMICS
Autore:
ISMAIL NH; COHN EJ; MOLLITT DL;
Indirizzi:
UNIV FLORIDA,HLTH SCI CTR JACKSONVILLE,DEPT SURG,655 W 8TH ST JACKSONVILLE FL 32209 UNIV FLORIDA,HLTH SCI CTR JACKSONVILLE,DEPT SURG JACKSONVILLE FL 32209
Titolo Testata:
The American surgeon
fascicolo: 1, volume: 63, anno: 1997,
pagine: 20 - 23
SICI:
0003-1348(1997)63:1<20:NSINSA>2.0.ZU;2-X
Fonte:
ISI
Lingua:
ENG
Soggetto:
BLOOD-CELL DEFORMABILITY; SEPSIS SYNDROME; RED;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
24
Recensione:
Indirizzi per estratti:
Citazione:
N.H. Ismail et al., "NITRIC-OXIDE SYNTHASE INHIBITION NEGATES SEPTIC-INDUCED ALTERATIONS IN CYTOPLASMIC CALCIUM HOMEOSTASIS AND MEMBRANE DYNAMICS", The American surgeon, 63(1), 1997, pp. 20-23

Abstract

This study was undertaken to evaluate the role of nitric oxide (NO) in the sepsis-induced disruption of intracellular calcium homeostasis and membrane dynamics. Anticoagulated whole blood was obtained from 10 healthy volunteers. Equal aliquots were incubated with saline (control), 2 mu g/mL Escherichia coli endotoxin (lipopolysaccharide), 8 mu g/mL NO inhibitor, N-monomethyl arginine (NMA), and endotoxin plus NO inhibitor (lipopolysaccharide/NMA). Erythrocytes were harvested, washed, and loaded with the calcium chelator, FURA-2AM, and the fluorescent membrane probe TMA-DPH. Cells were evaluated for both intracellular calcium concentration and membrane viscosity (anisotropy) by fluorescent spectrophotometry. Endotoxin induced a significant increase in both intracellular calcium concentration and anisotropy. NMA had no intrinsic affect on either of these cellular characteristics. NMA was, however, effective in preventing the endotoxin-induced changes. These results suggest that NO may play a role in the disruption of intracellular calcium homeostasis and erythrocyte membrane deformability noted in sepsis.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/07/20 alle ore 03:06:43