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Titolo:
UP-REGULATION OF UROKINASE-TYPE PLASMINOGEN-ACTIVATOR IN SQUAMOUS-CELL CARCINOMA OF HUMAN LARYNX
Autore:
PAROLINI S; FLAGIELLO D; CINQUETTI A; GOZZI R; CRISTINI S; CAPPIELLO J; NICOLAI P; RUSNATI M; PRESTA M; TOSATTI M;
Indirizzi:
UNIV BRESCIA,UNIT HISTOL & CYTOL,DEPT BIOMED SCI & BIOTECHNOL,VIA VALSABBINA 19 I-25123 BRESCIA ITALY UNIV BRESCIA,UNIT HISTOL & CYTOL,DEPT BIOMED SCI & BIOTECHNOL I-25123BRESCIA ITALY UNIV BRESCIA,UNIT PATHOL & IMMUNOL,DEPT BIOMED SCI & BIOTECHNOL I-25123 BRESCIA ITALY UNIV BRESCIA,SCH MED,OTORHINOLARYNGOL CLIN I-25123 BRESCIA ITALY
Titolo Testata:
British Journal of Cancer
fascicolo: 8, volume: 74, anno: 1996,
pagine: 1168 - 1174
SICI:
0007-0920(1996)74:8<1168:UOUPIS>2.0.ZU;2-7
Fonte:
ISI
Lingua:
ENG
Soggetto:
FIBRONECTIN MESSENGER-RNA; HUMAN-PROSTATE TISSUES; HUMAN BREAST-CANCER; HUMAN-COLON; INSITU HYBRIDIZATION; IMAGE-ANALYSIS; QUANTITATIVE-EVALUATION; PARTIAL-PURIFICATION; ADENOMATOUS POLYPS; URINARY-BLADDER;
Keywords:
LARYNX CARCINOMA; UROKINASE; IN SITU HYBRIDIZATION; IMMUNOHISTOCHEMISTRY; METASTASIS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
47
Recensione:
Indirizzi per estratti:
Citazione:
S. Parolini et al., "UP-REGULATION OF UROKINASE-TYPE PLASMINOGEN-ACTIVATOR IN SQUAMOUS-CELL CARCINOMA OF HUMAN LARYNX", British Journal of Cancer, 74(8), 1996, pp. 1168-1174

Abstract

The expression of urokinase-type plasminogen activator (uPA) was investigated in squamous cell carcinoma of the human larynx. For this purpose, tissue extracts from 25 matched samples of normal mucosa and neoplastic larynx were compared for the levels of uPA activity as evaluated by a chromogenic PA assay and sodium dodecyl sulphate-polyacrylamidegel electrophoresis (SDS-PAGE) zymography. Also, uPA antigen was quantified by enzyme-linked immunosorbent assay (ELISA) in 19 cases. The results demonstrate a significant increase in the levels of uPA activity and protein in tumour tissue extracts, more pronounced in rumours with lymph node metastases. Immunohistochemistry performed on 70 biopsies showed that uPA positivity is present both in neoplastic cells and in fibroblast-like cells and macrophages. However, depending on the histological grading and invasive capacity of the tumour, a pronounced intra- and intertumoral heterogeneity in uPA staining was observed. In situ hybridisation confirmed the presence of uPA mRNA in both tumour and stromal cells. The present study provides experimental evidence for a role of uPA in the invasive growth of human laryngeal carcinoma.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/10/20 alle ore 15:32:00