Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
COUNTERACTING THE MUTAGENIC EFFECT OF HYDROLYTIC DEAMINATION OF DNA 5-METHYLCYTOSINE RESIDUES AT HIGH-TEMPERATURE - DNA MISMATCH N-GLYCOSYLASE MIG.MTH OF THE THERMOPHILIC ARCHAEON METHANOBACTERIUM-THERMOAUTOTROPHICUM THF
Autore:
HORST JP; FRITZ HJ;
Indirizzi:
UNIV GOTTINGEN,INST MOL GENET,GRISEBACHSTR 8 D-37077 GOTTINGEN GERMANY UNIV GOTTINGEN,INST MOL GENET D-37077 GOTTINGEN GERMANY
Titolo Testata:
EMBO journal
fascicolo: 19, volume: 15, anno: 1996,
pagine: 5459 - 5469
SICI:
0261-4189(1996)15:19<5459:CTMEOH>2.0.ZU;2-0
Fonte:
ISI
Lingua:
ENG
Soggetto:
ESCHERICHIA-COLI K-12; RESTRICTION-MODIFICATION SYSTEM; MALTOSE-BINDING PROTEIN; ENZYME ENDONUCLEASE-III; G-A MISPAIRS; DEOXYRIBONUCLEIC-ACID; CYTOSINE RESIDUES; MOLECULAR-CLONING; REPAIR ENZYME; HELA-CELLS;
Keywords:
DNA METHYLATION; DNA MISMATCH REPAIR; HYDROLYTIC DNA DEAMINATION; SPONTANEOUS MUTATION; THERMOSTABLE ARCHAEAL PROTEIN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
50
Recensione:
Indirizzi per estratti:
Citazione:
J.P. Horst e H.J. Fritz, "COUNTERACTING THE MUTAGENIC EFFECT OF HYDROLYTIC DEAMINATION OF DNA 5-METHYLCYTOSINE RESIDUES AT HIGH-TEMPERATURE - DNA MISMATCH N-GLYCOSYLASE MIG.MTH OF THE THERMOPHILIC ARCHAEON METHANOBACTERIUM-THERMOAUTOTROPHICUM THF", EMBO journal, 15(19), 1996, pp. 5459-5469

Abstract

Spontaneous hydrolytic deamination of DNA 5-methyl-cytosine residues gives rise to T/G mismatches which are pre-mutagenic lesions requiringDNA repair, For fundamental reasons, the significance of this and other processes lowering genetic fidelity must be accentuated at elevatedtemperatures, making thermophilic organisms attractive objects for studying how cells cope with thermal noise threatening the integrity of their genetic information, Gene mig of Methanobacterium thermoautotrophicum THF, an anaerobic archaeon with an optimal growth temperature of65 degrees C, was isolated and its product (Mig.Mth; EC3,2,2-) shown to be a T/G-selective DNA thymine N-glycosylase with the properties required for counteracting the mutagenic effect of hydrolytic 5-meC deamination, The enzyme acts on T/G and U/G oppositions with similar efficiency; G/G, A/G, T/C and U/C are minor substrates; no other oppositionof common nucleobases is attacked and no removal of U from single-stranded DNA is observed, Substrate preferences are modulated by sequencecontext. Together with the results presented here, one example of an enzyme directed against the hydrolytic deamination damage of 5-meC is known from each of the three phylogenetic kingdoms; entry into the repair pathway is glycosylytic in the eukaryotic and the archaeal case, whereas the eubacterial repair starts with an endonucleolytic DNA incision.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/09/20 alle ore 12:09:04