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Titolo:
DOES PRECONDITIONING ACT BY GLYCOGEN DEPLETION IN THE ISOLATED RAT-HEART
Autore:
KING LM; OPIE LH;
Indirizzi:
UNIV CAPE TOWN,SCH MED,HEART RES UNIT,ANZIO RD ZA-7925 CAPE TOWN SOUTH AFRICA UNIV CAPE TOWN,SCH MED,MRC,ISCHAEM HEART DIS RES UNIT ZA-7925 CAPE TOWN SOUTH AFRICA
Titolo Testata:
Journal of Molecular and Cellular Cardiology
fascicolo: 12, volume: 28, anno: 1996,
pagine: 2305 - 2321
SICI:
0022-2828(1996)28:12<2305:DPABGD>2.0.ZU;2-A
Fonte:
ISI
Lingua:
ENG
Soggetto:
POSTISCHEMIC CONTRACTILE DYSFUNCTION; HYPOTHERMIC ISCHEMIC ARREST; LEFT-VENTRICULAR FUNCTION; INFARCT SIZE LIMITATION; ENERGY-METABOLISM; GLOBAL-ISCHEMIA; RABBIT HEART; GLYCOLYTIC INHIBITION; MYOCARDIAL PROTECTION; TIME-COURSE;
Keywords:
PRECONDITIONING; GLYCOLYSIS; CONTRACTURE; IRREVERSIBLE INJURY; METABOLITE ACCUMULATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
51
Recensione:
Indirizzi per estratti:
Citazione:
L.M. King e L.H. Opie, "DOES PRECONDITIONING ACT BY GLYCOGEN DEPLETION IN THE ISOLATED RAT-HEART", Journal of Molecular and Cellular Cardiology, 28(12), 1996, pp. 2305-2321

Abstract

Preconditioning hastens the time to onset of ischaemic contracture and increases peak contracture in an isolated perfused rat heart, but improves recovery of function. The preconditioning ischaemic episode is also known to deplete glycogen stores. We tested whether a depletion in glycogen is related to the protection conferred by preconditioning. The isolated Langendorff perfused rat heart, with a left ventricular balloon to record function, was perfused with either glucose 11 mM, acetate 5 mM, or glucose 11 maa + insulin to alter pre-ischaemic glycogenlevels prior to 30 min total global ischaemia. In addition, hearts were preconditioned by an episode of 5 min ischaemia and 5 min reperfusion. Time to onset of contracture (TOC-min), peak contracture and recovery of developed pressure after 20 min reperfusion with glucose-containing perfusate (both expressed as percentage pre-ischaemic developed pressure) were measured (n=9-10). Parallel groups of hearts were clamped at various times for assessment of tissue metabolites. Acetate pre-perfusion reduced glycogen levels compared to glucose hearts, from 16.27 +/- 0.44 to 10.77 +/- 0.96 mu mol/g wet wt. TOC was reduced and peak: contracture increased, with poor functional recovery, Glucose + insulin pre-perfusion increased glycogen (21.39 +/- 1.08 mu mol/g wet wt) with opposite effects on contracture, but functional recovery was still poor. Preconditioning hastened the time to onset of contracture, which could be partially attributed to glycogen depletion. Preconditioning significantly improved functional recovery in glucose hearts, but had little or no effect in the other groups. Thus the protective effect on functional recovery could not be linked to glycogen depletion. Pre-ischaemic glycogen appeared to play a dual role. When low, preconditioning was ineffective, presumably because of lack of production of glycolytic ATP, and severe contracture. When pre-ischaemic glycogen was increased, preconditioning was also relatively ineffective, presumably because of excess accumulation of the metabolites of glycogenolysis. (C) 1996 Academic Press Limited

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Documento generato il 03/12/20 alle ore 15:59:07