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Titolo:
LI(RB-86) TRANSPORT HETEROGENEITY IN THE LOW-DENSITY FRACTION OF SICKLE-CELL-ANEMIA RED-BLOOD-CELLS
Autore:
ETZION Z; LEW VL; BOOKCHIN RM;
Indirizzi:
ALBERT EINSTEIN COLL MED,DEPT MED,1300 MORRIS PK AVE BRONX NY 10461 ALBERT EINSTEIN COLL MED,DEPT MED BRONX NY 10461 PHYSIOL LAB CAMBRIDGE CB1 3EG ENGLAND
Titolo Testata:
American journal of physiology. Cell physiology
fascicolo: 4, volume: 40, anno: 1996,
pagine: 1111 - 1121
SICI:
0363-6143(1996)40:4<1111:LTHITL>2.0.ZU;2-A
Fonte:
ISI
Lingua:
ENG
Soggetto:
ION-CONTENT REGULATION; K-CL COTRANSPORT; VOLUME; RETICULOCYTES; DEHYDRATION; MODEL; ERYTHROCYTES; MEMBRANE; CALCIUM; CATION;
Keywords:
ERYTHROCYTES; ION TRANSPORT; BUMETANIDE; OUABAIN; POTASSIUM; RB-86;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
23
Recensione:
Indirizzi per estratti:
Citazione:
Z. Etzion et al., "LI(RB-86) TRANSPORT HETEROGENEITY IN THE LOW-DENSITY FRACTION OF SICKLE-CELL-ANEMIA RED-BLOOD-CELLS", American journal of physiology. Cell physiology, 40(4), 1996, pp. 1111-1121

Abstract

Previous studies have suggested ion transport heterogeneity among sickle cell anemia (SS) reticulocytes that could influence their dehydration susceptibility. We examined Ca2+-independent K trans port in the lowest density (F1), reticulocyte-rich SS cells, measuring the effects of acidification, ouabain. and bumetanide on their unidirectional K(Rb-86) fluxes. Unlike those of normal red blood cells and SS discocytes,the SS-F1 K(Rb-86) fluxes were highly nonlinear, with large 5-min flux components (previously unobserved) and a more gradual decline over 60 min. Analysis revealed two distinct K pools: a rapid-turnover pool in a small fraction of cells, whose major ouabain-resistant K(Rb-86) transport path showed distinctive properties including inhibition by high concentrations of bumetanide (greater than or equal to 1 mM) and stimulation at pH 7.0, and another heterogeneous, relatively slow-turnover pool, in most of the Fl cells, whose main ouabain-resistant K(Rb-86)path was insensitive to bumetanide but was stimulated at pH 7.0, which is consistent with heterogeneous expression of the acid-sensitive K-Cl cotransport and with both rapid and slower generation of dehydratedSS cells.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 19/09/20 alle ore 09:06:59