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Titolo:
CULTURE AND CHARACTERIZATION OF HUMAN NASAL GLAND-CELLS
Autore:
FURUKAWA M; YAMAYA M; IKEDA K; OSHIMA T; SUZUKI T; SASAKI H; TAKASAKA T;
Indirizzi:
TOHOKU UNIV,SCH MED,DEPT OTORHINOLARYNGOL,AOBA KU,1-1 SEIRYO MACHI SENDAI MIYAGI 98077 JAPAN TOHOKU UNIV,SCH MED,DEPT GERIATR MED,AOBA KU SENDAI MIYAGI 98077 JAPAN
Titolo Testata:
American journal of physiology. Lung cellular and molecular physiology
fascicolo: 4, volume: 15, anno: 1996,
pagine: 593 - 600
SICI:
1040-0605(1996)15:4<593:CACOHN>2.0.ZU;2-V
Fonte:
ISI
Lingua:
ENG
Soggetto:
ISOLATED ACINAR-CELLS; GUINEA-PIG; NONALLERGIC INDIVIDUALS; ALLERGIC PATIENTS; PATHO-PHYSIOLOGY; ION-TRANSPORT; MUCOSA; ACETYLCHOLINE; EPITHELIUM; SECRETION;
Keywords:
ACETYLCHOLINE; CHLORIDE SECRETION; USSING CHAMBERS; INTRACELLULAR CALCIUM ION CONCENTRATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
33
Recensione:
Indirizzi per estratti:
Citazione:
M. Furukawa et al., "CULTURE AND CHARACTERIZATION OF HUMAN NASAL GLAND-CELLS", American journal of physiology. Lung cellular and molecular physiology, 15(4), 1996, pp. 593-600

Abstract

To study the regulation of ion transport by human nasal gland (HNG) cells, we developed a system for culturing HNG cells isolated by enzymatic digestion from human nasal polyps. When plated on flasks in media containing Ultroser G serum substitute and a variety of growth factors, the HNG cells became confluent after 5-7 days. Cells were collected by trypsinization and replated at 10(6) cells/cm(2) on porous filters. Confluent monolayers formed on days 3-5 after replating and were studied on days 5-7. Transepithelial resistance and short-circuit current (I-sc) were 177 +/- 15 Omega . cm(2) and 12.2 +/- 1.3 mu A/cm(2) (means +/- SE, n = 35). Acetylcholine (ACh, 10(-5) M) induced a transient increase in I-sc by 4.4 +/- 1.0 mu A/cm(2) when added to both apical and basolateral sides and returned to baseline levels within 5 min. Diphenylamine -2 - carboxylate (10(-3) M) decreased baseline I-sc by 30.2 /- 1.7% (n = 6) and inhibited I-sc responses induced by ACh. Amiloride also decreased baseline I-sc by 12.3 +/- 3.4% (n = 6) but failed to inhibit ACh-induced I-sc responses. ACh (10(-5) M) also induced an increase in intracellular Ca2+ concentration measured by fura 2-AM from 60.5 +/- 16.3 to 248.3 +/- 45.3 nM (n = 4). These findings suggest thatgland cells from human nasal polyps can be grown in culture to produce epithelial cell sheets of high resistance, which secrete Cl- in response to cholinergic agent via an increase in intracellular Ca2+ concentration.

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Documento generato il 09/07/20 alle ore 18:05:05