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Titolo:
SHORT-TERM INSULIN-TREATMENT PREVENTS THE DIABETOGENIC ACTION OF STREPTOZOTOCIN IN RATS
Autore:
THULESEN J; ORSKOV C; HOLST JJ; POULSEN SS;
Indirizzi:
UNIV COPENHAGEN,PANUM INST,INST MED ANAT,DEPT B,BLEGDAMSVEJ 3 DK-2200COPENHAGEN N DENMARK UNIV COPENHAGEN,PANUM INST,INST MED ANAT & MED PHYSIOL DK-2200 COPENHAGEN DENMARK
Titolo Testata:
Endocrinology
fascicolo: 1, volume: 138, anno: 1997,
pagine: 62 - 68
SICI:
0013-7227(1997)138:1<62:SIPTDA>2.0.ZU;2-0
Fonte:
ISI
Lingua:
ENG
Soggetto:
DNA STRAND BREAKS; GLUCOSE-TRANSPORTER; PANCREATIC-ISLETS; POLY(ADP-RIBOSE) SYNTHETASE; GENE-EXPRESSION; BETA-CELLS; B-CELL; SEQUENCE; REGENERATION; GLICENTIN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
34
Recensione:
Indirizzi per estratti:
Citazione:
J. Thulesen et al., "SHORT-TERM INSULIN-TREATMENT PREVENTS THE DIABETOGENIC ACTION OF STREPTOZOTOCIN IN RATS", Endocrinology, 138(1), 1997, pp. 62-68

Abstract

Streptozotcin, which induces diabetes mellitus in experimental animals, has been reported to be taken up by beta-cells by means of the glucose transporter 2 (GLUT2) and then reduce the cellular level of NAD(+), leading to necrosis of the beta-cells. We investigated the effect ofinsulin pretreatment on the diabetogenic action of streptozotocin (60mg/kg). Four groups of rats were studied: 1) a group that received streptozotocin (STZ), 2), a group that received insulin pretreatment andstreptozotocin (INS + STZ), 3) a group that received insulin (INS), and 4, a control group (CTRL). Insulin treatment reduced the beta-cell immunoreactivity (IR) of insulin and GLUT2, which, thus, was reduced in INS + STZ rats at the time at the time of streptozotocin injection. In STZ rate, plasma insulin concentrations after 3 weeks as well as insulin concentrations in pancreatic tissue samples were significantly lower than those in CTRL rats [plasma. 274.3 +/- 101.9 vs. 1078.8 +/- 254.9 pmol/liter (P < 0.05); tissue, 0.46 +/- 0.02 vs. 117.0 +/- 28.4 nmol/g (P < 0.01)]. INS + STZ rats: did not become hyperglycemic, and the plasma and tissue levels of insulin were higher than those in STZ rats [plasma, 538.3 +/- 80.1 us. 274.3 +/- 101.9 pmol/liter (P - 0.08);tissue, 0.46 +/- 0.02 vs. 37.90 +/- 2.13 nmol/g (P < 0.05)]. The immunohistochemical findings of insulin IR in the pancreatic tissues were in accordance with the results obtained by RIA. We conclude that exogenous insulin suppresses the expression of GLUT2 and insulin in beta-cells, and this may prevent the diabetogenic effect of streptozotocin.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/11/20 alle ore 04:53:22