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Titolo:
APO-B-100 SECRETION BY HEPG2 CELLS IS REGULATED BY THE RATE OF TRIGLYCERIDE BIOSYNTHESIS BUT NOT BY INTRACELLULAR LIPID POOLS
Autore:
BENOIST F; GRANDPERRET T;
Indirizzi:
LAB GLAXO WELLCOME,CTR RECH,25 AVE QUEBEC,ZA COURTABOEUF F-91951 LES ULIS FRANCE LAB GLAXO WELLCOME,CTR RECH F-91951 LES ULIS FRANCE
Titolo Testata:
Arteriosclerosis, thrombosis, and vascular biology
fascicolo: 10, volume: 16, anno: 1996,
pagine: 1229 - 1235
SICI:
1079-5642(1996)16:10<1229:ASBHCI>2.0.ZU;2-J
Fonte:
ISI
Lingua:
ENG
Soggetto:
APOLIPOPROTEIN-B SECRETION; DENSITY-LIPOPROTEIN RECEPTOR; OLEIC-ACID; APO-B; MESSENGER-RNA; A-I; MICROSOMAL TRIGLYCERIDE; ENDOPLASMIC-RETICULUM; TRANSFER PROTEIN; DIETARY-FAT;
Keywords:
LIPOPROTEIN ASSEMBLY; CHOLESTERYL ESTER; HEPATOCYTE; 5-TETRADECYLOXY-2-FURANCARBOXYLIC ACID;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
44
Recensione:
Indirizzi per estratti:
Citazione:
F. Benoist e T. Grandperret, "APO-B-100 SECRETION BY HEPG2 CELLS IS REGULATED BY THE RATE OF TRIGLYCERIDE BIOSYNTHESIS BUT NOT BY INTRACELLULAR LIPID POOLS", Arteriosclerosis, thrombosis, and vascular biology, 16(10), 1996, pp. 1229-1235

Abstract

Triglycerides (TGs), cholesteryl esters (CEs), cholesterol, and phosphatidylcholine have been independently proposed as playing regulatory roles in apoB-100 secretion; the results depended on the cellular model used. In this study, we reinvestigate the role of lipids in apoB-100production in HepG2 cells and in particular, we clarify the respective roles of intracellular mass and the biosynthesis of lipids in the regulation of apoB-100 production. In a first set of experiments, the pool size of cholesterol, CEs, and TGs was modulated by a 3-day treatment with either lipid precursors or inhibitors of enzymes involved in lipid synthesis. We used simvastatin (a hydroxymethylglutaryl coenzyme Areductase inhibitor), 58-035 (an acyl coenzyme A cholesterol acyltransferase inhibitor), 5-tetradecyloxy-2-furan-carboxylic acid (TOFA, an inhibitor of fatty acid synthesis), and oleic acid. The secretion rateof apoB-100 was not affected by the large modulation of lipid mass induced by these various pretreatments. In a second set of experiments, the same lipid modulators were added during a 4-hour labeling period. Simvastatin and 58-035 inhibited cholesterol and CE synthesis without affecting apoB-100 secretion. By contrast, treatment of HepG2 cells with TOFA resulted in the inhibition of TG synthesis and apoB-100 secretion. This effect was highly specific for apoB-100 and was reversed by adding oleic acid, which stimulated both TG synthesis and apoB-100 secretion. Moreover, a combination of oleic acid and 58-035 inhibited CE biosynthesis and increased both TG synthesis and apoB-100 secretion. These results show that in HepG2 cells TG biosynthesis regulates apoB-100 secretion, whereas the rate of cholesterol or CE biosynthesis has no effect.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/03/20 alle ore 19:00:59